伊托必利对肝衰竭大鼠胃肠道动力的影响

Itopride effect on gastrointestinal motility in rats with hepatic failure

摘要:

目的  观察伊托必利对肝衰竭大鼠胃肠道动力的影响，探讨其可能的作用机制。方法  60只Wistar大鼠随机分为肝衰竭模型组（以下简称模型组）、肝衰竭模型+伊托必利治疗组（以下简称治疗组）和对照组，采用葡聚糖蓝-2000为胃肠内标记物，测定大鼠胃肠道传输的改变，通过放射免疫和免疫组织化学法观察大鼠胃窦、小肠组织中胃动素（MTL）的含量和分布变化。结果  与对照组比较，模型组大鼠胃肠道动力明显减弱（P <0.01），胃窦、十二指肠组织中MTL的含量及分布无明显减少（P >0.05）；但伊托必利治疗后，大鼠胃肠道传输明显改善（P <0.05），胃、十二指肠组织中MTL的含量和分布明显升高，与模型组比较差异有统计学意义（P <0.05）。结论  伊托必利能明显改善肝衰竭大鼠的胃肠道功能，其机制可能与MTL有关。

Abstract:

Objective To observe the effect of Itopride on gastrointestinal motility in rats with hepatic fai-lure, and to explore its possible mechanism. Methods Sixty Wistar rats were randomly divided into hepatic failure model group (model group), the hepatic failure model + Itopride therapy group (treatment group) and normal control group. Blue dextran-2000 was used as the gastrointestinal marker. Gastrointestinal transit in rats was determined. Radioimmunoassay and immunohistochemistry method were used to observe the changes of content and distribution of motilin (MTL) in gastric antrum and small intestine of the rats. Results Compared with the control group, the gastrointestinal motility of the rats in the model group significantly decreased (P < 0.01), the content and distribution of MTL in the gastric antrum and duodenum tissue did not significantly decreased (P > 0.05). However, after Itopride treatment, gastrointestinal transmission was significantly improved in the rats (P < 0.05), the content and distribution of MTL increased significantly in the tissues of stomach and duodenum, there were significant differences compared with the model group (P < 0.05). Conclusions Itopride hydrochloride can significantly improve gastrointestinal function of the rats with hepatic failure, and its mecha-nism may be related to MTL.