基质金属蛋白酶-2和E-钙黏蛋白在胰腺癌组织中的表达及临床意义

Expressions of MMP-2 and E-cadherin in prancreatic cancer tissues and their clinical significance

摘要:

目的  研究基质金属蛋白酶-2（MMP-2）及E-钙黏蛋白（E-cad）在胰腺癌组织中的表达及临床意义。方法  选取2011年6月-2013年6月该院收集的手术石蜡标本89例，其中正常胰腺组织标本34例，胰腺癌组织标本55例，采用免疫组织化学法检测MMP-2及E-cad的表达。结果  胰腺癌组织MMP-2阳性率为52.7%，正常组织MMP-2阳性率为2.9%，差异有统计学意义（P <0.05），胰腺癌组织MMP-2阳性率高于正常组织。胰腺癌组织E-cad阳性率为32.7%，正常组织MMP-2阳性率为100.0%，差异有统计学意义（P <0.05），胰腺癌组织E-cad阳性率低于正常组织。MMP-2及E-cad的表达与临床分期、组织学分化、是否有淋巴结转移及肿瘤直径有关（P <0.05），而与患者性别、年龄及肿瘤部位无关（P >0.05）。MMP-2及E-cad蛋白表达阳性2例，阴性10例，两者表达呈负相关（r =-0.673，P =0.027）。结论  MMP-2表达上调和E-cad表达下调与胰腺癌的发生、发展、转移关系密切。临床上可通过检测MMP-2和E-cad表达对胰腺癌的发展和转移进行预测和评估。

Abstract:

Objective To study the expression and clinical significance of matrix metalloprotenase 2 (MMP-2) protein and E-cadherin (E-cad) in pancreatic cancer tissues. Methods From June 2011 to June 2013, 89 tissue specimens were collected in our hospital, including 34 specimens of normal pancreatic tissue and 55 specimens of pancreatic cancer tissues. The expressions of MMP-2 and E-cad proteins were detected by immunohistochemical technique. Results The positive rate of MMP-2 in the pancreatic cancer tissues was 52.7% which was significantly higher than 2.9% of the normal tissue (P < 0.05). The positive expression rate of E-cad in the pancreatic cancer tissues was 32.7% which was significantly lower than 100.0% in the normal tissue (P < 0.05). The expressions of MMP-2 and E-cad were correlated to clinical stage, histological differentiation, lymph node metastasis and tumor diameter (P < 0.05), but were not correlated to gender, age or tumor location (P > 0.05). MMP-2 and E-cad protein expressions were positive in 2 cases, negative in 10 cases; the MMP-2 and E-cad expressions were negatively correlated (r = -0.673, P = 0.027). Conclusions The up-regulation of MMP-2 expression and down-regulation of E-cad expression in pancreatic cancer tissues are closely correlated with the occurrence, development and metastasis of pancreatic cancers. The development and metastasis of pancreatic cancers can be predicted and evaluated by detection of MMP-2 and E-cad expressions.