程序性细胞死亡4 mRNA在结直肠癌组织中的表达及意义

Expression of PDCD4 mRNA in human colorectal cancer and its significance

摘要:

目的  研究腹腔镜结直肠癌根治术癌组织中程序性细胞死亡4（PDCD4）mRNA的表达，以及与结直肠癌临床资料的相关性。方法  收集2012～2014年腹腔镜手术结直肠癌及癌旁正常组织标本54例，采用逆转录-聚合酶链反应（RT-PCR）检测结直肠标本中PDCD4 mRNA水平；分析PDCD4 mRNA水平与临床资料的相关性。结果  ①结直肠癌组织中PDCD4表达降低甚至不表达，表达降低率为64.8%（35/54），△Ct值为（4.50±0.60）；正常组织中PDCD4高表达，△Ct值为（3.54±0.52），差异有统计学意义（P <0.05）。②结直肠癌组织中PDCD4 mRNA水平与分化程度、有无淋巴结转移、肿瘤浸润深度有关，与性别、年龄、肿瘤部位无关；分化程度越低，有淋巴结转移，浸润越深，则PDCD4 mRNA表达更低。结论  结直肠癌中PDCD4 mRNA表达下降，PDCD4 mRNA水平与性别、年龄、肿瘤部位无关，与分化程度、有无淋巴结转移、肿瘤浸润深度有关。

Abstract:

 Objective To study programmed cell death 4 (PDCD4) mRNA expression in colorectal cancer tissues from laparoscopic radical resection, as well as its relationship with the clinical data of colorectal cancer. Methods Specimens of colorectal cancer tissues and normal tissues adjacent to carcinoma from 54 cases were collected in laparoscopic surgery between 2012 and 2014. PDCD4 mRNA level was detected by RT-PCR in colorectal cancer specimens. The relationship between PDCD4 mRNA level and clinical data was analyzed. Results PDCD4 expression in the colorectal cancer tissues reduced or even was not seen, the rate of lowered PDCD4 expression was 64.8% (35/54) with △Ct (4.50 ± 0.60); the normal tissues had high expression of PDCD4 with △Ct (3.54 ± 0.52), the difference was statistically significant (P < 0.05). The PDCD4 mRNA level in the colorectal cancer tissues was correlated with the differentiation degree, lymph node metastasis and tumor infiltration depth, but not correlated with gender, age or tumor location. PDCD4 mRNA expression was lower in patients with lymph node metastases than those without ymph node metastasis. The lower the degree of differentiation and the deeper infiltration, the lower the PDCD4 mRNA expression. Conclusions PDCD4 mRNA expression lowers in colorectal cancers. PDCD4 mRNA level is not correlated with gender, age or tumor location, but correlated with degree of differentiation, lymph node metastasis and tumor infiltration depth.