白细胞介素-24基因在膝关节骨性关节炎发病中的作用及意义

Expression of IL24 gene in knee osteoarthritis and its significance

摘要:

目的  探讨白细胞介素-24（IL-24）基因在膝关节骨性关节炎（KOA）疾病中的作用及意义，为KOA的早期诊断及基因治疗提供一定的科学依据。方法  选择该医院骨科原发性KOA的滑膜标本，提取总RNA，用实时逆转录聚合酶链反应（Real-time RT-PCR）检测KOA滑膜组织中IL-24基因的表达。结果  IL-24基因表达在KOA滑膜组织中降低（P <0.01）。结论  IL-24在膝关节骨性关节炎滑膜中的表达降低，且与KOA病变关系密切，为将来诊断和基因治疗膝关节骨性关节炎疾病提供参考。

Abstract:

Objective To investigate expression and significance of IL24 gene in the knee osteoarthritis (KOA), and to provide scientific basis for early diagnosis and gene therapy for knee osteoarthritis. Methods Fresh synovial specimens were collected from orthopedic patients in our hospital. Trizol was used to extract total RNA. Expression of IL24 in knee osteoarthritis synovium was detected by RT-PCR. Results IL24 gene expression in knee KOA synovial tissue obviously decreased (P < 0.01). Conclusions IL24 expression in the synovium of osteoarthritis knee joint obviously declines and is negatively correlated with the progress of KOA. Therefore, it can provide a reference for future diagnosis and gene therapy of knee osteoarthritis.