黄芩对急性重症胰腺炎大鼠肝损伤的预防及治疗作用研究

Protective and treatment effects of Scutellaria baicalensis on injured hepatic tissue of rats with acute severe pancreatitis

摘要:

目的  研究黄芩（Bai）对急性重症胰腺炎（ASP）大鼠肝损伤的预防及治疗作用。方法  选取Sprague Dawley大鼠36只，随机分为正常组、ASP组、制模前应用黄芩ASP组（Bai前ASP组）、制模后应用黄芩ASP组（Bai后ASP组）、制模前后均应用ASP组（Bai前后ASP组）、头孢哌酮ASP组（抗生素ASP组）。制模给药24和48 h后进行血淀粉酶（AMY）、谷丙转氨酶(ALT）、白细胞介素-6（IL-6）和核转录因子-κB（NF-κB）活性检测，分析肝组织病理学改变。通过上述指标的改变分析黄芩对ASP大鼠疾病进程的影响。结果  ASP组AMY、ALT、IL-6和NF-κB活性明显高于其他组，肝细胞变性，肝小叶中央静脉出血明显。Bai前后ASP组上述指标仅高于正常组，肝脏组织病变程度最轻。Bai前ASP组与抗生素组的治疗效果和病情发展变化程度相近；24 h时，Bai前ASP组和抗生素组的治疗效果和病情发展优于Bai后ASP组，但发病48 h时不及Bai后ASP组。结论  黄芩对ASP大鼠肝损伤有预防和治疗作用，可以有效减轻肝、胰组织损伤程度。本研究所用剂量（ASP前预防性给3 g黄芩/100g体重；ASP后给予5 g黄芩/100 g体重）对ASP大鼠肝损伤有治疗作用。

Abstract:

 Objective To study the preventive and treatment effectsof Scutellaria baicalensis on injured hepatic tissue of rats with acute severe pancreatitis (ASP). Methods Thirty-six Sprague Dawley rats were randomly divided into normal group (normal), ASP group, Bai-pre-ASP group (using Scutellaria baicalensis before acute severe pancreatitis model), Bai-post-ASP (using Scutellaria baicalensis after acute severe pancreatitis model), Bai-pre post-ASP group (using Scutellaria baicalensis before and after acute severe pancreatitis model), and Cef-ASP group (using Cefoperazone after acute severe pancreatitis model). The blood AMY, ALT, IL-6 and nuclear factor (NF)-кB were detected 24 and 48 h after the drugs were given to the rats. The hepatic tissues were also analyzed. The effects of Scutellaria baicalensis on the procedure of acute severe pancreatitis in rats were investigated. Results The activity of AMY, ALT, IL-6 and NF-кB in the ASP group was obviously higher than other groups. Degeneration of hepatic cells, hemorrhage of central veins of hepatic lobules were apparent. The above indexes of the Bai-pre post-ASP group were higher than those of the normal group, but lower than those of other treatment groups. The treatment effect in the Bai-pre-ASP group was similar to that of the Cef-ASP group, and they were better than that of the Bai-post-ASP group in 24 h. However, they were worse than those of the Bai-post-ASP group in 48 h. Conclusions Scutellaria baicalensis has the effects of prevention and treatment on the liver injure of the rats with acute severe pancreatitis. Our research showed that the usage of Scutellaria baicalensis 3 g per 100 g weight before ASP and 5 g per 100 g weight after ASP is appropriate for treatment of liver injury in ASP rats.