硬皮病动物模型建模药物最适浓度探讨

Optimal drug concentration for scleroderma mouse model

摘要:

目的  探讨用博来霉素（BLM）制作硬皮病动物模型的最适浓度。方法  将24只BALB/C小鼠随机平均分为3个实验组和1个对照组，各实验组小鼠分别皮下注射200、700和1 000μg/ml浓度的BLM，对照组小鼠皮下注射等量的磷酸盐缓冲液（PBS），注射4周后处死小鼠，取注射部位皮肤行苏木精-伊红染色法（HE）染色、Masson染色、真皮羟脯氨酸含量检测及真皮厚度检测。结果  700和1 000μg/ml浓度的BLM成功建模，但1 000μg/ml的浓度可导致小鼠皮肤浅表溃疡。结论  硬皮病动物模型成功与否与BLM药物浓度高低关系密切，700μg/ml博莱霉素浓度具有较好的建模效果，且不会出现皮肤溃疡的副作用。

关键词:

硬皮病;博来霉素;动物模型;最适浓度

Abstract:

Objective To analyze the optimal concentration of Bleomycin for scleroderma mouse model. Methods Twenty-four BALB/c mice were divided into 4 groups including 3 experimental groups and 1 control group. The mice of the experimental groups were injected with 200, 700 and 1,000 μg/ml Bleomycin respectively and those in the control group were injected with PBS. The staining for HE and Masson, and detection for dermis hydroxyproline content and dermis thickness were executed after the extraction of injective-site skin about 4 weeks later. Results Scleroderma mouse model was successfully established at the 700 and 1,000 μg/ml concentration of Bleomycin, but the skin ulcer of injective site occured in the experimental group of 1,000 μg/ml concentration. Conclusions There is a close relationship between establishment of scleroderma mouse model and drug concentration. The 700 μg/ml concentration of Bleomeycin has a good molding effect, which does not have the side effect like skin ulcer.

Key words:

scleroderma; Bleomycin; animal model; optimal concentration