水药酢浆草对四氯化碳致小鼠肝损伤的保护性研究

Protective effect of Shui ethnic medicine oxalis corniculata on liver injured by carbon tetrachloride in mice

摘要:

目的 探讨水药酢浆草对四氯化碳CCl4诱导的小鼠肝损伤的保护作用。方法 将40只小鼠随机分为5组，正常组、CCl4模型组、酢浆草低、中、高剂量组，每组8只。正常组和CCl4模型组给予生理氯化钠溶液，酢浆草低、中、高剂量组给分别给100、300及600 mg/kg酢浆草灌胃，连续5 d。于末次给药2 h后，除正常组外，其余各组小鼠给予0.3% CCl4花生油溶液稀释、按0.02 ml/g的剂量皮下注射，在中毒后16 h取血和肝脏组织，分别检测各组小鼠肝功能相关的生化指标。结果 与CCl4模型组小鼠比较，不同剂量酢浆草治疗组小鼠肝脏指数、血清ALT、AST及TNF-α水平不同程度降低（P <0.05）；肝组织中CAT、GSH及SOD水平不同程度升高（P <0.05），MDA水平不同程度降低（P <0.05）。结论 水药酢浆草对CCl4所致小鼠肝损伤具有明显的保护作用，其机制可能与酢浆草降低氧化酶活性和抗脂质过氧化作用有关。

Abstract:

【Objective】 To investigate the liver protective effect of Shui ethnic medicine oxalis corniculata on hepatic injury induced by carbon tetrachloride (CCl4) in mice. 【Methods】 Forty mice were randomly divided into normal group, CCl4 model group, low-dose oxalis corniculata group, medium-dose oxalis corniculata group and high-dose oxalis corniculata group with 8 in each group. Normal group and CCl4 model group were given normal saline; low-, medium- and high-dose oxalis corniculata groups were orally given 100, 300 and 600 mg/kg oxalis corniculata respectively for 5 consecutive days. Except the normal group, other groups were given 0.3% CCl4 (diluted with peanut oil solution) subcutaneously at a 0.02 ml/g dose 2 h after the last administration. Blood and liver tissue were collected 16 h later to detect biochemical indicators related to liver function. 【Results】 Compared with the CCl4 model group, serum ALT, AST and TNF-α levels in the oxalis corniculata groups decreased (P < 0.05); the levels of CAT, GSH and SOD significantly increased and the content of MDA remarkably reduced to different extent in liver homogenate of the oxalis corniculata groups (P < 0.05). 【Conclusions】 Shui ethnic medicine oxalis corniculata has a good liver protective effect on the hepatic injury induced by CCl4 in mice, and the mechanism is probably related with the decrease of liver anti-oxidative enzyme activities and the anti-lipid-peroxidation effect.