Th1/Th2/Th17细胞在实验性过敏性结膜炎中的作用研究

Effect of Th1/Th2/Th17 balance on development of experimental allergic conjunctivitis

摘要:

目的  探讨Th1/Th2/Th17细胞在实验性过敏性结膜炎发病中的可能作用。方法  40只Brown Norway大鼠随机分为3组：空白组、对照组和实验组。应用鸡卵清蛋白（OVA）复制大鼠过敏性结膜炎模型。取3组大鼠眼球及上下眼睑进行病理学分析，计数穹隆部结膜嗜酸性粒细胞浸润数。ELISA法测定大鼠血清IgE、IgG1、IgG2a和脾细胞培养上清液IL-17、IL-4、IL-6、IL-10、IFN-γ含量。流式细胞仪测定大鼠外周血和脾脏Th17细胞。结果  实验组大鼠穹隆部眼结膜嗜酸性粒细胞浸润数较空白组和对照组升高（P <0.01）。实验组大鼠血清IgE、IgG1较空白组和对照组升高（P <0.01），血清IgG2a较空白组和对照组降低（P <0.01）。实验组大鼠血清脾细胞培养上清液IL-17、IL-4、IL-6、IL-10水平较空白组和对照组升高（P <0.01），IFN-γ水平较空白组和对照组降低（P <0.01）。实验组大鼠外周血和脾脏Th17细胞均高于空白组和对照组（P <0.01）。结论  Th1/Th2/Th17细胞平衡失衡是大鼠实验性过敏性结膜炎发病的主要原因。恢复Th1/Th2/Th17失衡可能成为过敏性结膜炎治疗的一条新途径。

Abstract:

Objective To investigate the effect of Th1/Th2/Th17 balance on development of experimental allergic conjunctivitis. Methods Forty Brown Norway rats were randomly divided into three groups: normal group, control group and experimental allergic conjunctivitis group (experimental group). Ovalbumin (OVA)-
induced allergic conjunctivitis models were established. The eyes including eyelids and conjunctivae were harvested for histological analysis, and infiltrating eosinophils in fornical conjunctivae were counted. The serum levels of IgE, IgG1 and IgG2a and the concentrations of IL-17, IL-4, IL-6, IL-10 and IFN-γ in spleen culture supernatant were measured by ELISA. The Th17 cells in peripheral blood and spleen tissue were detected using flow cytometry. Results The experimental group had significantly higher number of conjunctival eosinophils than the normal and control groups (P < 0.01). The experimental group had significantly higher serum IgE and IgG1 levels and significantly lower serum IgG2a level than the normal and control groups (P < 0.01). The concentrations of IL-17, IL-4, IL-6 and IL-10 in spleen culture supernatant were significantly higher but the IFN-γ level was significantly lower in the experimental group than in the normal and control groups (P < 0.01). The Th17 cells in the peripheral blood and spleen tissue of the experimental group were significantly more than those of the normal and control groups (P < 0.01). Conclusions Th1/Th2/Th17 imbalance might play an important role in the development of experimental allergic conjunctivitis. To modulate the unbalanced Th1/Th2/Th17 reaction may contribute to treatment of allergic conjunctivitis.