Objective To study the clinical value of e-flow imaging in evaluation of artificial arteriovenous fistula. Methods The function of the arteriovenous fistula was measured by e-flow imaging along with routine color Doppler flow image (CDFI) in 115 patients with hemodialysis. Results There were 79 cases of internal fistula patency, 10 cases of stenosis, 18 cases of thrombosis and 8 cases of phlebangioma ectasia. E-flow image clearly displayed blood flow and background tissue at the same time, when combined with CDFI it raised the rate of abnormal image display. Conclusions E-flow imaging is a new and reliable means for diagnosis of artificial arteriovenous fistula.