microRNA-221在结直肠癌中的表达及其对癌细胞迁移的影响

Expression of microRNA-221 in colorectal carcinoma and its impact on cancer cells' migration

摘要:

目的  研究上皮-间质转化（EMT）相关的微小RNA（microRNA，以下简称miRNA）-221在结直肠癌患者中的表达水平，并探索其对结直肠癌细胞迁移水平的影响。方法  选取该院40例结直肠癌患者为研究组，选取同期体检的40例健康人群为对照组。采用实时定量RT-PCR法检测结直肠癌患者癌组织以及癌旁组织中miRNA-221表达水平，同时对比结直肠癌患者及正常人血浆中miRNA-221表达水平。对结直肠细胞采用miRNA-221的mimic及inhibitor分别高、低表达miRNA-221后，采用transwell法分别检测细胞的侵袭水平以及EMT相关蛋白表达。结果  miRNA-221在直肠癌组织的表达水平显著高于癌旁组织，差异有统计学意义（P <0.05）；miRNA-221在直肠癌患者血浆中的表达水平显著高于健康对照人群，差异有统计学意义（P <0.05）。干扰结直肠癌HT-29细胞株中miRNA-221后，结直肠癌细胞迁移能力显著降低（均P <0.05），且细胞中EMT相关的上皮钙黏蛋白（E-cad）的表达水平降低；而过表达HT-29细胞株中miRNA-221，结直肠癌细胞迁移能力显著增加，且细胞中EMT相关的上皮钙黏蛋白（E-cad）的表达水平增加。结论  结直肠癌患者高表达的miRNA-221通过促进结直肠癌细胞的迁移水平来参与结直肠癌发生发展的进程。

Abstract:

Objective To study miRNA-221 expression in colorectal tissue and plasma of cancer patients, and to explore the mechanism about its migration of colorectal cancer. Methods 40 cases of colorectal cancer patients in our hospital as the study group,40 cases of healthy people as the control group, we used RT-PCR to detect the mRNA levels in peripheral blood or tissue between of two groups; in addition, after overexpression or knockdown of microRNA-221, we detected the migration of cancer cells. Results The expression of microRNA-221 in colorectal cancer tissues was significantly higher than the adjacent tissues (P ＜ 0.05); in addition, the expression of microRNA-221 in plasma of colorectal cancer patients was significantly higher than the normal population (P ＜ 0.05). Knockdown of microRNA-221 in colorectal cancer cell line reduced cell invasion and E-cad expression significantly (P ＜ 0.05); whereas overexpression of microRNA-221 in colorectal cancer cell line improved cell invasion and E-cad expression significantly (P ＜ 0.05). Conclusion High expression of microRNA-221 in colorectal cancer patients participated in the development of colorectal cancer by promoting the migration of colorectal cancer cells.