Gadd45α、NF-κB在胃癌中表达情况及其相关性研究

Differential expression and correlation of Gadd45α and NF-κB in human gastric cancer

摘要:

目的  通过分析Gadd45α、NF-κB在不同幽门螺杆菌（HP）感染状态胃癌中的差异表达来探讨两者表达的相关性及其对胃癌发生的意义。方法  随机选取本院70例胃癌石蜡标本及56例相应癌旁正常组织标本来检测Gadd45α、NF-κB蛋白的表达情况；分析Gadd45α蛋白与NF-κB在胃癌中表达的相关性。结果  NF-κB在HP+胃癌、HP-胃癌、HP+癌旁组织中的阳性表达率分别为82.5%、53.33%和26.32%，Gadd45α蛋白在HP+胃癌、HP-胃癌、HP+癌旁组织中的阳性表达率分别为77.5%、46.67%和34.21%，经过χ2检验比较阳性表达的差异有统计学意义（P <0.05）。Gadd45α与NF-κB在胃癌中表达呈正相关（P <0.05）。结论  NF-κB、Gadd45α蛋白在胃癌组织中高表达，且Gadd45α与NF-κB蛋白两者在胃癌中的表达呈正相关。

Abstract:

Objective To explore the differential expression level of Gadd45α and NF-κB in human gastric cancer with Helicobacter Pylori (HP) infection status, and to explore a way to clinical diagnosis and treatment for gastric cancer. Methods A total of 70 paraffin-emeded samples of gastric cancer and 56 normal tissues from surgical resected tissues were selected randomly. We analyzed the correlation between the expression of Gadd45α protein and NF-κB in gastric carcinoma. Results The positive rates of expression of NF-κB protein in HP+ gastric carcinoma tissues, HP-gastric carcinoma tissues and HP+ adjacent non-neoplastic tissues were 82.5%, 53.33% and 26.32% respectively. The positive rates of expression of Gadd45α protein in HP+ gastric carcinoma tissues, HP-gastric carcinoma tissues and HP+ adjacent non-neoplastic tissues were 77.5%, 46.67% and 34.21% respectively. The difference of the expression levels was significant (P < 0.05 ). The expression of Gadd45α protein in gastric carcinoma samples was positively correlated with NF-κB (P < 0.05 ). Conclusions The levels of Gadd45α and NF-κB protein expression are up-regulated in HP+ gastric carcinoma sample. The expression of Gadd45α and NF-κB in gastric carcinoma is positively correlated.