Caspase-3、DIAPH-3蛋白在浸润性乳腺癌中的作用研究

Role of caspase-3 and DIAPH3 in invasive breast cancer

摘要:

目的  探讨浸润性乳腺癌组织中Caspase-3、DIAPH-3蛋白的表达与临床病理特征及预后的关系。方法  采用免疫组织化学EliVision法检测Caspase-3、DIAPH-3在浸润性乳腺癌（n =240）及癌旁组织（n =44）中的表达情况，并分析其与临床病理特征及预后的关系。结果  Caspase-3、DIAPH-3在浸润性乳腺癌组织中表达的阳性率（36.7%和40.8%）低于癌旁组织（81.8%和86.7%），P <0.05。Caspase-3的阳性表达率随淋巴结转移数的增多、肿瘤长径的增加、临床分期的提高、脉管瘤栓的产生及P53的阳性表达的增强而降低。DIAPH-3的表达随淋巴结转移数的增加、脉管瘤栓的产生及临床分期的提高而降低。Kaplan-Meier单因素生存分析显示Caspase-3和DIAPH-3蛋白阳性组患者的5年生存率（95.5%、95.8%），高于阴性组（85.5%、84.5%）。COX多因素生存分析显示DIAPH-3阴性表达与患者不良预后正相关（■=1.687，95%CI：1.032，2.758）。结论  浸润性乳腺癌组织中Caspase-3、DIAPH-3蛋白表达均低于癌旁正常乳腺组织，两者均与乳腺癌的进展及预后密切相关，但两者之间无关联；DIAPH-3的低表达提示患者预后不良，可能是预测乳腺癌患者预后的独立因素之一。

Abstract:

Objective To study the possible association of the expressions of caspase-3 and diaphanous related formin 3 (DIAPH3) protein with clinicopathological features and prognosis in invasive breast cancer. Methods The expressions of caspase-3 and DIAPH3 protein in invasive breast cancer tissues from 240 cases and para-cancerous tissues from 44 cases were detected by immunohistochemical EliVision method, and then their relationships with the clinicopathological features and prognosis were analyzed. Results The positive-expression rates of caspase-3 and DIAPH3 protein in the invasive breast cancer tissues (36.7% and 40.8% respectively) were significantly lower than those in the para-cancerous tissues (81.8% and 86.7% respectively, P ＜ 0.05). The expression of caspase-3 was negatively correlated with the number of lymph node metastasis, tumor size, advanced tumor clinical stage, vascular tumor embolus and positive expression of P53. The expression of DIAPH3 was also negatively correlated with the number of lymph node metastasis, vascular tumor embolus and advanced tumor clinical stage. Kaplan-Meier single factor analysis showed that the 5-year survival rates in the patients with positive expressions of caspase-3 and DIAPH3 were 95.5% and 95.8% respectively, significantly higher than those in the negative-expression patients (85.5% and 84.5% respectively). The Cox model analysis showed that the negative expression of DIAPH3 might be a risk factor for the prognosis of invasive breast cancer (■ = 1.687, 95%CI: 1.032, 2.758). Conclusions The negative expressions of caspase-3 and DIAPH3 protein are important for the development and prognosis of  invasive breast cancer, but there is no correlation between them. The low expression of DIAPH3 is probably one of the independent factors of prognosis in breast cancer.