MircoRNA-195在胃癌患者血清及组织中的表达水平及意义

Expression of mircoRNA-195 in serum and tissues of patients with gastric cancer and its significance

摘要:

目的  研究microRNA-195（miR-195）在患者血清及胃癌组织中的表达水平，并探讨其表达水平与胃癌临床病理的关系。方法  选取该院62例胃癌患者为研究组，同期来该院体检的36例健康人群为对照组，采用RT-PCR法检测两组人群血清中以及胃癌组织及对应癌旁组织中的miR-195的表达水平，并探讨miR-195与临床病理参数的相互关系。结果  研究组血清中miR-195的表达水平显著低于对照组，差异有统计学意义（P <0.05）。miR-195在胃癌组织中的表达水平亦显著低于对应的癌旁组织，差异有统计学意义（P <0.05）；相关分析结果显示，miR-195的低表达与胃癌浸润深度、分化程度、临床分期及淋巴结转移相关，差异有统计学意义（P <0.05），而与肿瘤部位均无相关（P >0.05）。结论  miR-195在胃癌患者中的低表达可能对胃癌的发生发展有一定的促进作用。

Abstract:

Objective To study the expression of mircoRNA-195 (miR-195) in serum and tissues of patients with gastric cancer, and to discuss its correlation with clinical pathology of gastric cancer. Methods Sixty-two patients with gastric cancer in our hospital were selected as research group, and 36 healthy people who were accepted for physical examination in our hospital in the same period as control group. The expression level of miR-195 in serum, gastric cancer tissue and corresponding para-carcinoma tissue of the two groups was detected by real-time PCR; and the correlations between the expression level of miR-195 and clinicopathological parameters were discussed. Results The expression level of miR-195 in serum of the research group was significantly lower than that of the control group (P < 0.05). The expression level of miR-195 in the gastric cancer tissue was significantly lower than that of the corresponding para-carcinoma tissue (P < 0.05). Correlation analysis showed that the low expression level of miR-195 was significantly correlated with the invasion depth, differentiation degree, clinical stages and lymphatic metastasis of gastric cancer (P < 0.05), but it had no significant correlation with tumor location (P > 0.05). Conclusions The low expression level of miR-195 has certain effect on promoting the occurrence and development of gastric cancer.