热休克蛋白27、60和90在胃癌中的表达及其临床价值

Expression of HSP-27, 60 and 90 in gastric cancer and its clinical significance

摘要:

目的  探讨热休克蛋白（HSP-27）、HSP-60和HSP-90在胃癌中的表达及其临床意义。方法  采用免疫组织化学法检测66例胃癌组织HSP-27、HSP-60和HSP-90的表达，并结合临床病理特征、肿瘤增殖能力和患者生存期，分析3种热休克蛋白（HSPs）表达的临床意义。结果  HSP-27、HSP-60和HSP-90在胃癌组织中异常高表达。HSP-27表达与肿瘤大小、器官转移及病理分期相关，而HSP-27染色强度与淋巴转移显著相关。HSP-60表达与患者性别相关，而HSP-60染色强度与患者年龄和肿瘤病理组织学分级相关。HSP-90染色强度与肿瘤大小显著相关（P =0.020）。单因素分析表明，HSP-90高表达与生存期更长显著相关，多因素分析证实HSP-90高表达是胃癌独立预后因素。结论  HSP-27、HSP-60和HSP-90与具有治疗胃癌疗效评估价值的临床病理参数相关。HSP-90高表达是胃癌的独立预后指标。

Abstract:

Objetives To evaluate the clinical significance of heat shock protein (HSP)-27, HSP-60 and HSP-90 expression in gastric carcinoma. Methods HSP-27, HSP-60 and HSP-90 expression was assessed immunohistochemically in tumoral samples of 66 gastric adenocarcinoma patients and was statistically analyzed in relation to various clinicopathological characteristics, tumor proliferative capacity and patients' survival. Results HSP-27, HSP-60, HSP-90 were abundantly expressed in the gastric adenocarcinoma tissues. HSP-27 expression was significantly associated with tumor size, the presence of organ metastases and pathological stage, while HSP-27 staining intensity was significantly correlated with nodal status. HSP-60 expression was significantly correlated with patients' sex, while HSP-60 staining intensity with patients' age and tumor histopathological grade. HSP-90 expression was not associated with any of the clinicopathological parameters examined; however, HSP-90 staining intensity was significantly associated with tumor size. High HSP-90 expression was significantly associated with longer overall survival time in univariate analysis, and was also identified as an independent prognostic factor in multivariate analysis. Conclusions HSP-27, HSP-60 and HSP-90 are correlated with certain clinicopathological parameters which are crucial for the management of gastric adenocarcinoma patients. HSP-90 expression may also be an independent prognostic indicator in gastric adenocarcinoma patients.