Micro RNA-132对结肠癌细胞生物学功能的作用

Eeffect of miR-132 on biological characteristics of colon cancer cells

摘要:

目的  探讨microRNA-132（miR-132）对结肠癌细胞系LOVO生物学功能的作用。方法  通过体外转染miR-132 mimic的方式进行获得性功能实验。以细胞计数检测（CCK-8）、平板克隆形成、流式细胞凋亡检测以及划痕实验分析miR-132过表达对LOVO细胞增殖与侵袭的影响。结果  LOVO细胞中，外源性过表达miR-132能够抑制其生长，且抑制率呈时间、浓度依赖性，50 nmol/L miR-132 mimic处理72 h后，抑制率达30%。流式细胞凋亡检测发现miR-132过表达诱导细胞凋亡。miR-132过表达可以抑制LOVO细胞体内的外克隆形成能力。体外平板克隆形成实验显示，miR-132过表达使LOVO细胞克隆形成率从21%降至7%。划痕修复实验结果显示，高表达miR-132各组细胞的迁移能力降低。结论  miR-132过表达能显著抑制结肠癌细胞LOVO发生细胞凋亡及增殖，还能削弱结肠癌细胞LOVO的克隆形成能力。

Abstract:

 Objective To investigate the effect of miR-132 on biological characteristics of conlon cancer LOVO cell line. Methods Synthetic miR-132 mimics and miRNAs cramble were transfected into LOVO cells using Lipofectamine 2000. Transwell assay was used to assess the effect of miR-132 on LOVO cell invasion and metastasis. CCK-8 assay was used to assess the effect of miR-132 on LOVO cell proliferation. Flow cytometry was used to assess the effect of miR-132 on LOVO apoptosis. Results Over-expression of miR-132 induced LOVO cell apoptosis, and subsequently inhibited LOVO cell growth and colony formation. Notably, 50 nmol/L miR-132 mimic demonstrated a potent inhibitory effect at 72 h after transfection and reduced cell viability by 30%. The colony formation ability was impared after miR-132 mimic treatment with a 14% drop of in vitro colony formation rate. Also, miR-132 inhibited the LOVO cell migration. Conclusions miR-132 could suppress colon cancer cell growth through induction of cell apoptosis.