钛合金表面纳米氧化锌薄膜的体外细胞毒性研究

In vitro cytotoxicity study of nano-ZnO thin film on surface of titanium alloy

摘要:

目的  评价钛合金表面纳米氧化锌薄膜对细胞毒性的作用。方法  观察小鼠胚胎成纤维L-929细胞在含纳米氧化锌薄膜的钛合金析出液中的生长情况，采用噻唑蓝比色法测定各组细胞的吸光度值，并计算出相对的细胞增殖率，进行细胞毒性评价。结果  各组细胞生长状况良好，细胞形态无异常，增值率>80%，细胞毒性等级为1级。结论  钛合金表面纳米氧化锌薄膜对细胞生长无毒害作用。

关键词:

钛合金;纳米氧化锌;细胞毒性;噻唑蓝比色法;生物相容性

Abstract:

Objective To evaluate the cytotoxicity of nano-ZnO thin film on titanium alloy. Methods The growth of L-929 cells in the culture solutions consisting of the ions released from nano-ZnO thin film on the surface of titanium alloy was observed. And the absorbance values of the cells were tested by MTT method. The relative growth rate of L-929 cells was calculated and the cytotoxicity of nano-ZnO thin film on the surface of titanium alloy was evaluated. Results The relative growth rate of each L-929 cell group was above 80% and the cytotoxicity grade was grade 1. Conclusions Nano-ZnO thin film on the surface of titanium alloy has almost no cytotoxicity.

Key words:

titanium alloy; nano-ZnO; cytotoxicity; MTT method; biocompatibility