外周血中CD19+ CD5+ B细胞和白细胞介素-10对系统性红斑狼疮患者病情的影响

Expression and significance of CD19+ CD5+ B cells and IL-10 in patients with systemic lupus erythematosus

摘要:

目的  探讨外周血中CD19+ CD5+ B细胞和白细胞介素-10（IL-10）对系统性红斑狼疮（SLE）患者病情发生、发展的影响，为其临床研究提供参考依据。方法  选择65例稳定期SLE患者及65例活动期SLE患者，同时选择70例健康人群作为对照组，采用流式细胞术检测外周血中CD19+ CD5+ B细胞数量，采用酶联免疫吸附法检测IL-10水平。比较3组的差异，并分析上述指标与SLE活动度评分（SLEDAI）及补体的相关性。结果  活动组及稳定组CD19+ CD5+ B细胞数量、白蛋白、C3、C4均低于对照组，IL-10、红细胞沉降率（ESR）高于对照组，差异有统计学意义（P <0.05），活动组CD19+ CD5+ B细胞数量、白蛋白、C3、C4低于稳定组，SLEDAI评分、IL-10、ESR高于稳定组，差异有统计学意义（P<0.05）。CD19+ CD5+ B细胞数量与SLEDAI评分呈负相关，IL-10与SLEDAI评分呈正相关（P <0.05）。随着SLEDAI评分的增加，CD19+ CD5+ B细胞数量呈下降趋势，而IL-10水平呈上升趋势，差异有统计学意义（P <0.05）。结论  SLE患者外周血CD19+ CD5+ B细胞数量降低，而IL-10水平升高，随着病情活动程度的增加，外周血CD19+ CD5+ B细胞及IL-10水平变化更明显，可以作为评价SLE活动性的指标。

Abstract:

Objective To study the expressions of CD19+ CD5+ B cells and interleukin 10 (IL-10) and  their clinical significance in patients with systemic lupus erythematosus (SLE). Methods In this study 65 SLE-stable patients, 65 SLE-active patients and 70 healthy control people were included. CD19+ CD5+ B cell subsets were detected with flow cytometry. IL-10 level in serum was detected with enzyme linked immunosorbent assay (ELISA). The correlations of CD19+ CD5+ B cell count and serum IL-10 level with SLE disease activity index (SLEDAI) score and complement were analyzed. Results The CD19+ CD5+ B cell count, albumin, C3 and C4 in the SLE-active group and SLE-stable group were significantly lower than those in the control group (P < 0.05). The IL-10 and ESR in the SLE-active and stable groups were higher than those in the control group, the differences were statistically significant (P < 0.05). The CD19+ CD5+ B cell count, albumin, C3 and C4 in the SLE-active group were significantly lower than those in the SLE-stable group (P < 0.05). The SLEDAI score, IL-10 and ESR in the SLE-active group were higher than those in the SLE-stable group, the differences were statistically significant (P < 0.05). The CD19+ CD5+ B cell count was negatively related with the SLEDAI score, but IL-10 was positively related with the SLEDAI score (P < 0.05). As the SLEDAI score increased, CD19+ CD5+ B cell count showed a lowering trend, and IL-10 showed an uptrend.Conclusions CD19+ CD5+ B cells and IL-10 may participate in the occurrence and development of SLE. The changes of CD19+ CD5+ B cells and IL-10 in the peripheral blood might contribute to the pathogenesis and correlate with the disease activity of SLE, which can be used as evaluation indexes of SLE activity.