依达拉奉对高氧损伤小鼠肺组织中白介素-4、干扰素-γ、转化生长因子-β1的影响

Impact of Edaravone on hyperoxic lung injury in neonatal mice

摘要:

目的  探讨依达拉奉对新生小鼠高氧肺损伤的治疗作用，为临床防治新生儿高氧肺损伤提供理论依据。方法  将新生小鼠随机分为空气+生理盐水组、空气＋依达拉奉组、高氧+生理盐水组、高氧+依达拉奉组，于实验第3、7、10、14和21天取材，测定肺组织中白介素-4（IL-4）、干扰素-γ（IFN-γ）、肺组织中转化生长因子-β1（TGF-β1）表达的变化。结果  随着吸氧时间的延长，高氧组IL-4、IFN-γ含量均高于空气组且比例失衡，TGF-β1表达越多并肺组织结构紊乱；依达拉奉治疗组IFN-γ含量显著增高，IL-4含量明显降低，IFN-γ/IL-4比值更接近正常组，TGF-β1表达降低，肺组织结构较高氧组好转。结论  高氧可导致新生小鼠急性肺损伤；依达拉奉可调节IFN-γ、IL-4的含量及比例，降低TGF-β1的表达，发挥对高氧肺损伤的防治作用。

Abstract:

Objective To explore the effects of Edaravone in hyperoxic lung injury in neonatal mice so as to provide experimental and theoretical evidences for controlling hyperoxic lung injury in neonate. Methods Neonatal mice were randomly divided into air + normal saline, air + Edaravone, hyperoxia + normal saline, hyperoxia + Edaravone groups. At the end of exposure (on the 3rd, 7th, 10th, 14th and 21st day), IL-4 and IFN-γ in lung homogenate were evaluated by ELISA, and TGF-β1 and optical density (OD) in lung slices were determined using immunohistochemical stain and computerized graphic analysis techniques. Results With the increasing time of exposure, IL-4, IFN-γ and TGF-β1 of the hyperoxic group were increasing and higher than those in the air group, lung injury also aggravated. Compared to the hyperoxic group, the treatment group showed increased IFN-γ and decreased IL-4 and TGF-β1; histopathological changes were alleviated as well. Conclusions Hyperoxia can result in acute lung injury in neonatal mice. Edaravone can regulate the content and ratio of IFN-γ and IL-4, decrease the expression of TGF-β1, thus play a role in prevention and treatment of hyperoxic lung injury.