趋化因子CXCL14在甲状腺乳头状癌中的表达水平及其临床意义

Expression and significance of CXCL14 protein in papillary thyroid carcinoma

摘要:

目的  探讨趋化因子CXCL14在甲状腺乳头状癌中的表达及其与临床病理的关系。方法  采用免疫组织化学方法、蛋白印迹法（Western blot）检测120例甲状腺乳头状癌、40例结节性甲状腺肿及40例正常甲状腺组织中CXCL14的表达水平，并分析其与甲状腺乳头状癌的临床病理特征的关系。结果  免疫组织化学结果表明，甲状腺乳头状癌组织中CXCL14蛋白阳性率（20.83%）较结节性甲状腺肿（85.00%）、正常甲状腺组织（75.00%）明显降低，差异有统计学意义（P <0.05）。Western blot结果表明，CXCL14蛋白在甲状腺乳头状癌组织中的表达量为（0.823±0.143），明显低于结节性甲状腺肿组织（1.211±0.012）和正常甲状腺组织（1.219±0.004），差异有统计学意义（P <0.05）。CXCL14蛋白的表达与甲状腺乳头状癌的包膜浸润、淋巴结转移、肿瘤临床分期及肿瘤分化有关（P <0.05）。CXCL14高表达组的甲状腺乳头状癌患者的生存率明显高于CXCL14低表达组（P <0.05）。结论  CXCL14蛋白的表达可能成为判断甲状腺乳头状癌恶化程度及预后的指标。

Abstract:

Objective To analyze the expression of chemokine CXCL14 in papillary thyroid carcinoma and its correlation with clinicopathologic parameters. Methods Immunohistochemistry and Western blot were used to assess the expression of CXCL14 protein in 120 cases of papillary thyroid carcinoma, 40 cases of nodular goiter and 40 cases of normal tissues, and its association with clinicopathologic parameters was analyzed. Results Immunohistochemistry showed that the expression of CXCL14 was positive in 20.83% of papillary thyroid carcinoma tissue, which was significantly higher than that in nodular goiter (85.00%) and nomal tissue (75.00%) (P < 0.05 ). Western blot showed that the relative amount of CXCL14 protein in papillary thyroid carcinoma tissue was (0.823 ± 0.143) sig-
nificantly higher than in nodular goiter (1.211 ± 0.012) and normal tissues (1.219 ± 0.004) (P < 0.05). The expression of CXCL14 was correlated with envelope invasion, lymph node metastasis, clinical stage and differentiation (P < 0.05). The survival rate of patients with thyroid papillary carcinoma in high expression of CXCL14 was significantly higher than the low expression of CXCL14 (P < 0.05 ). Conclusions The expression of CXCL14 may be a marker for adjudging the malignancy and prognosis of papillary thyroid carcinoma.