决明子调脂作用的代谢组学研究

Metabonomic study on lipid regulative effect of Cassia

摘要:

目的  运用代谢组学方法考察决明子水提物对高脂血症大鼠血液内源性代谢物的影响。方法  建立高脂血症大鼠模型，采用决明子水提物灌胃治疗，利用主成分分析法和偏最小二乘-判别分析对空白对照组、模型组及治疗组大鼠血液进行代谢组学分析。观察3组代谢轮廓差异并寻找生物标志物。结果  利用主成分分析法和偏最小二乘-判别分析能将样本分开，谱库分析得到9种生物标志物：L-缬氨酸、丙氨酸、甘氨酸、富马酸、异亮氨酸、丝氨酸、硬脂酸、亚油酸和胆固醇。结论  基于气相色谱-质谱联用仪的代谢组学可以很好的从内源性代谢物的变化过程反应决明子水提物对大鼠的调脂作用。

Abstract:

Objective To study the influences of Cassia aqueous extract on endogenous metabolites of blood in rats with hyperlipemia by using metabonomic method. Methods The rats were fed on fat diet to induce hyperlipemia, then treated with cassia aqueous extract. We analyzed metabolic finger print data of rats' plasma in the control, model and treated group. The plasma samples underwent preparation, derivation and GC/MS analysis. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) was performed to detect the metabolic profile difference between the three groups. Results Through the metabonomic research, 9 biomarkers were found, which were L-valine, alanine, glycine, fumaric acid, isoleucine, leucine, serine, stearic acid, oleic acid, linoleic acid and cholesterol. Conclusions Metabonomic study based on GC-MS shows a good recognition of the lipid regulating effect of cassia aqueous extract.