膜联蛋白A1和CA19-9在胆管癌中的表达及其临床意义

Expressions of Annexin A1 and CA19-9 in cholangiocarcinoma and their clinicopathological significance

摘要:

目的  观察膜联蛋白A1（Annexin A1）和肿瘤特异性大分子糖蛋白抗原CA19-9在胆管癌中的表达，探讨两者与胆管癌临床病理特征之间的关系。方法  采用免疫组织化学检测54例胆管癌术后标本（癌组织和癌旁组织）及15例非肿瘤胆管组织中Annexin A1和CA19-9的表达，分析两者与临床病理特征的关系。采用酶联免疫吸附试验（ELISA）分别检测24例健康志愿者、24例胆管结石患者、24例胆管癌患者血清中Annexin A1和CA19-9的表达情况。结果  胆管癌组织中Annexin A1和CA19-9表达的阳性率显著高于癌旁组织和非肿瘤胆管组织（P <0.05）。Annexin A1的表达状态与胆管癌的临床病理分期、组织学分级及淋巴结转移有关（P <0.05），而与患者发病年龄、肿瘤大小无关（P >0.05）；CA19-9的表达与胆管癌的淋巴结转移有关（P <0.05），而与患者发病年龄、肿瘤大小、临床病理分期及组织学分级无关（P >0.05）。Annexin A1和CA19-9在胆管癌组织中的表达呈正相关（r =0.356，P <0.05）。ELISA结果表明，胆管癌患者组血清中Annexin A1和CA19-9表达含量高于胆管结石患者组及健康人组（P <0.05）。Annexin A1和CA19-9在胆管癌术前与术后血清中的表达差异有统计学意义（P <0.05）。结论  Annexin A1和CA19-9与胆管癌的发生、发展和淋巴结转移有关，联合检测可为胆管癌诊断及预后判断提供一定的参考价值。

Abstract:

Objective To investigate the expressions of Annexin A1 and CA19-9 in cholangiocarcinoma and their correlations with clinical and pathological features. Methods Immunohistochemistry was used to detect the expressions of Annexin A1 and CA19-9 in 54 samples of cholangiocarcinoma and the surrounding tissues and 15 samples of non-carcinoma bile duct tissue. Statistical analysis was performed with reference to the related clinical and pathological features. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expressions of Annexin A1 and CA19-9 in the serum of 24 healthy volunteers, 24 patients with bile duct stones and 24 cases of bile duct carcinoma. Results The positive expression rates of Annexin A1 and CA19-9 in the cholangiocarcinoma were significantly higher than those in the peri-carcinoma tissue and non-carcinoma bile duct tissue (P < 0.05). The positive expression of Annexin A1 was correlated with the pathological grades, clinical stages and lymph node metastasis of cholangiocarcinoma (P < 0.05). The positive expression of CA19-9 was correlated with the lymph node metastasis of cholangiocarcinoma (P < 0.05). The expression of Annexin A1 in the cholangiocarcinoma was positively correlated with that of CA19-9 (r = 0.356，P < 0.05). The results of ELISA indicated that the expressions of Annexin A1 and CA19-9 in serum of the cholangiocarcinoma patients were significantly higher than those in the patients with bile duct stones and the healthy volunteers (P < 0.05). In the cholangiocarcinoma patients, the expressions of Annexin A1 and CA19-9 before operation had significant differences from those after operation (P < 0.05). Conclusions The positive expressions of Annexin A1 and CA19-9 may be correlated with carcinogenesis, development and lymph node metastasis of cholangiocarcinoma. The combined detection of the above markers possibly has certain referential value in diagnosis of cholangiocarcinoma and its prognosis.