CT判断不同密度同种异体骨犬椎体间融合效果研究

CT in assessment of effect of lumbar interbody fusion after allotransplantation with different-density bones in dog

摘要:

目的  计算机断层扫描（CT）判断不同密度同种异体骨在犬椎体间融合情况，为临床椎体间植骨融合术中植入合适密度骨提供依据。方法  将21只健康中华田园犬随机分成3组，每组7只。所有动物均行经前路腰椎间盘摘除椎体间植骨融合术。3组分别予高密度植骨（甲组），正常密度植骨（乙组），空白对照（丙组）。于术后12周行腰椎CT平扫+三维重建观察椎间融合效果。应用Photoshop软件测量融合骨横截面CT图形面积，计算椎体间融合程度。结果  术后12周，3组椎体间融合的比例分别为甲组（75.91±8.25）、乙组（94.67±3.35）、丙组（37.78±1.16）。植骨组（甲组、乙组）与空白对照组（丙组）比较，差异有统计学意义（P <0.05），正常密度植骨组（乙组）椎间融合效果优于其他两组（P <0.05）。结论  椎体间融合术中移植未经压缩的同种异体骨，有利于促进椎体间融合。

Abstract:

Objective To assess the effect of application of allogeneic bones with different densities to canine's lumbar interbody fusion by CT, so as to provide clinical basis for such allotransplantation with appropriate-density allogeneic bones. Methods Twenty-one healthy Chinese pastoral dogs were randomly divided into 3 groups. All the dogs underwent anterior lumbar discectomy and interbody fusion. The three groups were treated with high-density bone allograft (group A), normal-density bone allograft (group B) and no graft (blank control group, group C). Lumbar CT scan and three-dimensional reconstruction were performed at the 12th week after operation to observe the effect of intervertebral fusion. Photoshop software was used to measure the area of the CT image of the cross section of newly-formed bones and calculate the degree of vertebral-body fusion. Results In the 12th week after surgery, the proportion of interbody fusion was (75.91 ± 8.25)% in the group A , (94.67 ± 3.35)% in the group B and (37.78 ± 1.16)% in the group C. There were statistically significant differences between the grafting bone groups (groups A and B) and the blank control group (group C) (P < 0.05). The effect of interbody fusion in the normal-density bone graft group (group B) was better than that of the other two groups (P < 0.05). Conclusions Transplantation of uncompressed allograft bone in interbody fusion can promote the fusion of the vertebral bodies.