维生素D受体基因的多态性与儿童喘息性疾病的关系

Relationships of vitamin D receptor gene polymorphisms with children asthma and wheezy bronchitis

摘要:

目的  探讨维生素D受体基因的多态性与儿童喘息性疾病的关系。方法  选取本院2012年9月-
2014年9月的82例喘息性疾病患儿进行分析。支气管哮喘41例，作为试验组1，喘息性支气管炎41例，作为试验组2，正常对照组41例，3组研究对象采用聚合酶链反应-限制性长度多态性检测维生素D受体ApaⅠ、FokⅠ、BsmⅠ和TaqⅠ基因多态性，观察3组研究对象的基因型分布频率及维生素D受体基因的多态性与儿童喘息性疾病的相关性。结果  试验组1与试验组2和对照组的维生素D受体ApaⅠ、FokⅠ、BsmⅠ和TaqⅠ基因型的分布频率之间存在明显差异，差异有统计学意义（P <0.05）；试验组1与试验组2和对照组的维生素D受体ApaⅠ、FokⅠ、BsmⅠ和TaqⅠ等位基因频率之间存在明显差异，差异有统计学意义（P <0.05）。结论  维生素D受体基因的多态性与儿童喘息性疾病具有良好的相关性，为治疗儿童喘息性疾病提供可靠依据。

Abstract:

Objective To study the relationship of vitamin D receptor gene polymorphisms with children asthma and wheezy bronchitis. Methods Eighty-two cases of asthma and wheezy bronchitis treated in our hospital from September 2012 to September 2014 were analyzed and divided into two groups: 41 cases of bronchial asthma were into experimental group 1, 41 cases of asthmatic bronchitis were into experimental group 2. There were 41 cases in normal control group. The three groups used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to detect vitamin D receptor ApaⅠ, FokⅠ, BsmⅠ and TaqⅠ gene polymorphisms. The frequency of genotype distribution of the 3 groups were observed and the correlations of the vitamin D receptor gene polymorphisms with children asthmatic diseases were studied. Results The frequency of vitamin D receptor ApaⅠ, FokⅠ, BsmⅠ and TaqⅠ genotype distribution in the experimental group 1 had significant differences from that in the group 2 and the normal control group (P < 0.05). The vitamin D receptor ApaⅠ, FokⅠ, BsmⅠ and TaqⅠ allele frequency of the experimental group 1 had significant differences from that of the group 2 and the normal control group (P < 0.05). Conclusions The vitamin D receptor gene polymorphisms are closely correlated with asthmatic diseases, which provides a reliable basis for treatment of children with asthmatic diseases.