白射干素对感染后咳嗽豚鼠细胞因子的影响

Influences of dichotomitin on cytokines in guinea pig with post infectious cough

摘要:

目的  观察白射干素对感染后咳嗽（PIC）豚鼠肺泡灌洗液中细胞因子的影响。方法  复制PIC豚鼠模型并收集支气管肺泡灌洗液（BALF），测定BALF中白细胞总数和分类计数，酶联免疫吸附试验测定细胞因子白介素-4（IL-4）和干扰素-γ（IFN-γ）水平。结果  模型组豚鼠肺泡灌洗液中白细胞总数、中性粒细胞、淋巴细胞数及IL-4水平较空白组升高（P <0.05）；IFN-γ水平较空白组降低（P <0.05）。白射干素干预组可明显降低PIC豚鼠BALF中白细胞总数、中性粒细胞、淋巴细胞及IL-4水平，同时升高IFN-γ水平，差异有统计学意义（P <0.05）。结论  提示白射干素可能通过调控细胞因子而减少模型豚鼠气道炎症细胞的浸润，发挥其抗炎、止咳作用。

Abstract:

Objective To study the effect of dichotomitin on cytokines in post infectious cough (PIC) model of guinea pig. Methods PIC guinea pig model was established, dichotomitin was given at the same time. Total WBC number, lymphocytes and neutrophils in bronchoalveolar lavage fluid (BALF) were detected. The levels of IFN-γ and IL-4 in BALF were detected by ELISA. Results In the mode group, the number of total WBC count, lymphocytes and neutrophils was increased, IL-4 level increased, IFN-γ level decreased (P < 0.05) in the BALF compared to the blank group. In the dichotomitin group, total WBC count, neutrophils, lymphocytes and the IL-4 level were obviously reduced, but IFN-γ level was increased (P < 0.05). Conclusions Dicho-tomitin might reduce infiltration of airway inflammatory cells of PIC model of guinea pig, and enhance the immunologic function.