缺氧微环境下人肺腺癌A549细胞的microRNA基因芯片结果分析

MicroRNA gene chip analysis of human lung adenocarcinoma A549 cells in anoxic microenvironment

摘要:

目的  研究缺氧对肺腺癌A549细胞中microRNA（miRNA）表达谱的影响，分析靶基因参与的生物学功能和通路。方法  基于miRNA芯片技术和生物信息学分析方法，分析缺氧条件和正常氧条件下培养的A549细胞中差异表达的miRNA，并预测该miRNA的靶基因，分析靶基因参与的生物学功能和通路。结果  本研究共筛选出14个差异表达miRNA，包括9个在缺氧细胞中上调miRNA和5个下调miRNA。上调和下调miRNA的靶基因都参与DNA转录、核染色质修饰等功能，并且都参与Wnt信号、转化生长因子-β信号和丝裂原活化蛋白激酶信号通路。结论  缺氧的肺腺癌A549细胞中miRNA表达谱发生显著改变，一些差异表达miRNA对某些基因具有调控作用。

Abstract:

Objective To analyze the effect of hypoxia on miRNA expression in lung adenocarcinoma A549 cells. Methods Based on miRNA chip technology and bioinformatic analysis methods, differential expression of miRNAs between hypoxia-treated A549 cells and normoxia-treated A549 cells were identified, and their target genes were predicted. Besides, biological functions and pathways of target genes were analyzed. Results In total, 14 differentially-expressed miRNAs were screened, including 9 upregulated miRNAs and 5 downregulated miRNAs in the hypoxia-treated A549 cells. Target genes of both upregulated and downregulated miRNAs were enriched in GO terms, such as DNA transcription, chromatin modification, as well as a set of pathways, such as Wnt signaling, TGF-beta signaling and MAPK signaling. Conclusions In the hypoxic A549 cells, miRNA expressions have significantly changed. A series of differentially-expressed miRNAs regulate certain genes.