柚皮苷对gp120所致BV2小胶质细胞损伤的保护作用

Protective effect of naringin on gp120-induced injury in BV2 microglial cells

摘要:

目的  探讨P2X7受体在HIV-1包膜糖蛋白gp120所致BV2小胶质细胞损伤中的作用，以及柚皮苷通过作用于P2X7受体对gp120所致BV2小胶质细胞损伤产生的保护作用。方法  通过gp120处理BV2小胶质细胞建立细胞损伤模型，并通过MTS比色法检测细胞损伤程度和柚皮苷是否对损伤细胞具有保护作用；应用逆转录PCR（RT-PCR）及蛋白质印迹法（Western blot）检测P2X7受体的表达变化。结果  gp120处理24 h后，与对照组比较，gp120组细胞存活率显著下降（P <0.01）；gp120+柚皮苷组的细胞存活率与gp120组比较有所上升（P <0.01），但与gp120+BBG组比较差异无统计学意义（P >0.05）；RT-PCR及Western blot的检测结果显示，gp120组小胶质细胞的P2X7受体mRNA及蛋白均比对照组明显升高（P <0.01），而gp120+柚皮苷组与gp120组比较有所下降（P <0.01）。结论  P2X7受体参与gp120所致BV2小胶质细胞损伤，柚皮苷可能通过抑制P2X7受体的表达上调对gp120所致细胞损伤产生保护作用。

Abstract:

Objective To explore the effect of P2X7 receptor on HIV-1 envelope glycoprotein gp120 (gp120)-induced injury in BV2 microglial cells, and research the protective effect of naringin on gp120-induced injury mediated by P2X7 receptor in BV2 microglial cells. Methods BV2 microglial cell injury model was established by gp120 treatment; The damage degree of BV2 microglial cells and the protective effect of naringin were measured by MTS colorimetry. The expression changes of P2X7 receptors were detected by RT-PCR and Western blot. Results Compared with Ctrl group, the cell survival rate of gp120 group decreased significantly after 24-h treatment (P < 0.01). The cell survival rate of gp120 + naringin group increased compared with gp120 group (P < 0.01), while there was no difference compared with gp120 + BBG group (P > 0.05). The results of RT-PCR and Western blot showed that the expression of P2X7 mRNA and protein in gp120 group was increased significantly compared with Ctrl group (P < 0.01), and it  was decreased in gp120 + naringin group compared with gp120 group (P < 0.01). Conclusions P2X7 receptor involved in gp120-induced injury in BV2 microglial cells, and naringin may play a protective effect on gp120-induced injury by inhibiting the up-regulation of P2X7 receptor expression.