Reprimo基因3'非翻译区839位点多态性与肺癌发生的关联

Study of Reprimo 839 G>C polymorphism and relationship with lung cancer

摘要:

目的  探讨Reprimo基因3'非翻译区839位点单核苷酸多态性改变与肺癌发生的关系。方法  基因分型用TAKARA的MightyAmp DNA 聚合酶法，分别检测36例肺癌患者（癌症组）和23例非癌患者（对照组）的Reprimo基因3'非翻译区839位点G >C分布，运用χ2检验、Logistic 回归分析和Armitage’s 趋势检验等分析基因多态性、患者性别、吸烟与否和年龄与肺癌发生的相关性。结果  两组标本经Hardy-Weinberg平衡检测，对照组在Reprimo基因3'非翻译区839位点G >C基因型观测(理论)频数为0，22，1（5.26，11.48，6.26），差异有统计学意义（P =0.000）,癌症组的基因型观测(理论)频数为6，29，1(11.67，17.65，6.67)，差异有统计学意义（P =0.000）；经Logistic 回归分析，基因多态性、年龄是危险因素，基因多态性、年龄和吸烟与否在肺癌发生中不存在交互作用（P >0.05）；经Armitage’s 趋势检验差异无统计学意义（χ2=3.56，P =0.059）。结论  Reprimo基因3'非翻译区839位点G >C单核苷酸位点多态性与肺癌发生具有一定的相关性。

Abstract:

Objective To investigate the relationship between the Reprimo gene 3'-untranslated region 839 polymorphism and lung cancer. Methods Genotyping was performed with MightyAmp DNA Polymerase, a number of 36 lung cancer cases and 23 control cases were genotyped. The results of investigation were analyzed with χ2 test, Logistic regression analysis and Armitage's trend test. Results According to Hardy-Weinberg equilibrium detection, Reprimo 839 G>C genotype observation (theory) frequency was 0, 22, 1 (5.26, 11.48, 6.26) in the control group (with statistically significant difference, P = 0.000) and 6, 29, 1 (11.67, 17.65, 6.67) in the cancer group (with statistically significant difference, P = 0.000). Analyzed by Logistic regression analysis, gene polymorphism and age were risk factors, and there were no possible interactions among gene polymorphism, age, smoking status and lung cancer (P > 0.05). Armitage's trend test revealed no statistically significant correlation between 839 G>C polymorphism and susceptibility to lung cancer (χ2 = 3.56, P = 0.059). Conclusions This study indicates that there is a correlation between the Reprimo gene 3'-untranslated region 839 G>C polymorphism and lung cancer.