促红细胞生成素后处理对大鼠肾脏缺血再灌注损伤的保护作用

Effect of erythropoietin postconditioning on renal ischemia-reperfusion injury in rats

摘要:

目的  探讨促红细胞生成素（EPO）后处理对大鼠肾脏缺血再灌注损伤（IRI）的保护作用。方法  将18只健康雄性SD大鼠随机分为假手术组（Sham组）、缺血再灌注损伤组（IRI组）及EPO后处理组（EPO组）。采用全自动生化分析仪检测血清尿素氮（BUN）及肌酐（Cr）含量；用HE染色观察病理组织学变化；用免疫组织化学法检测肾组织中HSP70的表达。结果  IRI组血清BUN及Cr含量较Sham组明显升高，EPO后处理组血清BUN及Cr含量介于Sham组和IRI组之间；HE染色检测发现IRI组肾小管上皮细胞坏死明显；肾小管管腔扩张，EPO后处理组较IRI组肾小管上皮坏死明显减轻；免疫组织化学检测发现HSP70在EPO后处理组表达最强，Sham组表达明显降低，IRI组表达则介于前两者之间。结论  EPO后处理能增强缺血再灌注损伤过程中HSP-70的表达量，因而有助于减轻肾脏的缺血再灌注损伤。

Abstract:

Objective To investigate the effect of erythropoietin (EPO) postconditioning on renal ischemia-reperfusion injury (IRI) in rats. Methods A total of 18 male Sprasue-Dawley (SD) rats were randomly allocated to three groups: Sham group, ischemia-reperfusion injury (IRI) group, EPO postconditioning group, respectively. Serum blood urea nitrogen (BUN) and creatinine (Cr) were analyzed with automatic biochemical analyzer. Histopathological changes were observed with HE staining. The expression of HSP70 in kidney was checked by immunohistochemistry. Results Serum BUN and serum Cr in IRI group were significantly higher than those in Sham group, while levels of serum BUN and serum Cr in EPO postconditioning group were between the Sham group and IRI group. Significant necrosis of tubular epithelial cells and tubular lumen expansion in hematoxylin were found in IRI group. Compared with IRI group, tubular necrosis significantly reduced in EPO postconditioning group. Immunohistochemistry showed that the expression of HSP70 in EPO postconditioning group was the strongest, and significantly decreased in the Sham group, and IRI group was between them. Conclusions EPO postconditioning enhances the expression of HSP70 after IRI, which helps to reduce kidney injury.