MACC1和CIP2A蛋白表达的相关性及其与胃癌预后的关系

Expressions of MACC1 and CIP2A proteins and their correlations with prognosis of gastric carcinoma

摘要:

目的  检测结肠癌转移相关基因1（MACC1）蛋白在胃癌病变组织及癌旁正常组织中的表达，以及与胃癌各临床病理特征的关系，进一步分析MACC1与蛋白磷酸酶2A的癌性抑制因子（CIP2A）蛋白表达是否具有相关性，评估胃癌预后，并为治疗提供思路。方法  应用逆转录聚合酶链反应检测胃癌组织和癌旁正常组织中MACC1蛋白的表达，分析其表达差异性。免疫组织化学法测定30例胃癌组织和癌旁正常组织中MACC1和CIP2A蛋白的表达，分析MACC1蛋白与胃癌临床不同病理特征的关系，同时分析MACC1和CIP2A蛋白的相关性。结果  MACC1蛋白在癌旁正常组织和胃癌组织中的阳性表达率分别为16.7%和76.7%，差异有统计学意义。CIP2A蛋白在癌旁正常组织和胃癌组织中的阳性表达率分别为23.3%和73.3%，差异有统计学意义。不同性别、年龄、组织类型的MACC1蛋白阳性表达率比较，差异无统计学意义；不同组织分化程度、肿瘤侵润程度、淋巴结转移程度、临床分期的的MACC1蛋白阳性表达率比较，差异有统计学意义；MACC1与CIP2A蛋白表达呈正相关。结论  MACC1与CIP2A蛋白呈正相关，两者联合检测可以判断患者的预后。

Abstract:

Objective To investigate the expression of metastasis-associated in colon cancer 1 (MACC1) protein in gastric cancerous tissue and adjacent noncancerous tissue and its relationships with the clinical pathological factors and the cancerous inhibitor of protein phosphatase 2A (CIP2A) protein expression, and evaluate the prognosis so as to provide the train of thought for the treatment of gastric cancer. Methods RT-PCR was used to assay the expression of MACC1 protein in gastric cancerous tissue and adjacent noncance-rous tissue. Immunohistochemical assay was used to detect the expressions of MACC1 and CIP2A proteins in gastric cancerous tissue and adjacent noncancerous tissue of 30 cases. The relationships of the expression of MACC1 protein with clinical pathological factors and CIP2A protein were analyzed. Results The positive rate of MACC1 in the gastric cancerous tissue and the adjacent noncancerous tissue was 16.7% and 76.7% respectively, the difference was statistically significant. The positive rate of CIP2A protein expression in the gastric cancerous tissue and the adjacent noncancerous tissue was 23.3% and 73.3% respectively with significant difference. The expression of MACC1 protein was not significantly different between the patients with different gender, different age or different histological types of gastric cancers. However, the patients with different depth of tumor invasion, different degree of cancer differentiation, different degree of lymph node metastasis and different clinical stage had significant differences in MACC1 expression. MACC1 and CIP2A protein expressions were positively correlated. Conclusions MACC1 protein expression is positively correlated with CIP2A protein expression, combined detection of both can help to determine the prognosis of the patients.