胆固醇代谢相关蛋白及相关特异microRNAs在胆固醇型胆囊结石患者血清中的表达及意义

Expressions and significance of cholesterol metabolism related proteins and miRNAs targeting to ABCA1 in serum of patients with cholesterol gallstones

摘要:

目的  探讨胆固醇型胆囊结石患者和健康对照人群血清中胆固醇代谢相关蛋白，以及可能靶向ABCA1的相关microRNAs（miRNAs）表达差异及意义。方法  选取60例胆固醇型胆囊结石患者（结石组）和60例健康对照人群（非结石组）的血清样本，采用Western blot检测胆固醇代谢相关蛋白（SREBP-2、FXR、LXR、ABCA1、ABCB11）的表达，利用生物信息学软件预测可能靶向调控ABCA1的相关miRNAs，采用实时荧光定量聚合酶链反应（RT-qPCR）对相关miRNAs的表达进行检测。结果  结石组血清中SREBP-2、FXR、LXR、ABCA1、ABCB11蛋白的表达量较非结石组降低（P <0.05），结石组血清中miRNA-26a、miRNA-144、miRNA-758表达较非结石组升高（P <0.05）。结论  胆固醇代谢相关蛋白差异性表达及可能靶向ABCA1的相关miRNA在胆固醇型胆囊结石的发病过程中可能发挥极为重要的作用。

Abstract:

Objective To investigate the expressions of serum cholesterol metabolism related proteins and miRNAs maybe targeting to ABCA1 in the cholesterol gallstone patients and control subjects and the significance. Methods Serum samples from 60 patients with cholesterol gallstones (GS) and 60 without gallbladder stone (GP) were collected. The expressions of serum cholesterol metabolism related proteins (SREBP-2, FXR, LXR, ABCA1 and ABCB11) were examined by Western blot assay. The miRNAs possibly targeting to ABCA1 were predicted by bioinformatics method, the expressions of the selected miRNAs were detected by real time-quantitative polymerase chain reaction (RT-qPCR). Results SREBP-2, FXR, LXR, ABCA1 and ABCB11 expressions in serum were significantly decreased in the GC patients compared to those in the healthy controls (P < 0.05). The serum expressions of miRNA-26a, miRNA-144 and miRNA-758 were significantly higher in the GC patients than in healthy controls (P < 0.05). Conclusions Abnormal expressions of cholesterol metabolism related proteins and the differential levels of microRNAs targeting to ABCA1 may play an important role in occurrence and development of cholesterol gallstones.