细胞周期突变体相关蛋白激酶2、P-糖蛋白和谷胱甘肽巯基转移酶-π在宫颈癌中的表达及临床意义

Expressions of Nek2, P-gp and GST-π in cervical cancer and their clinical significance

摘要:

目的  检测宫颈癌中细胞周期突变体（NIMA）相关蛋白激酶（Nek2）与耐药蛋白P-糖蛋白（P-gp）、胱甘肽巯基转移酶-π（GST-π）的表达及其与临床病理特征的相关性。方法  应用免疫组织化学法检测144例宫颈癌组织和32例慢性宫颈炎非癌组织中Nek2、P-gp、GST-π的表达。结果  ①Nek2、P-gp和GST-π在宫颈癌及宫颈炎组织中的阳性表达率分别为（51.4% vs 31.2%）、（55.6% vs 12.5%）和（73.6% vs 53.1%），组间比较差异有统计学意义（P <0.05）。②Nek2表达与宫颈癌组织学分级、临床分期相关（P <0.05），与年龄、组织学类型、浸润深度、细胞角蛋白（CK）、CK14、癌胚抗原（CEA）、抗癌基因P16、生存时间无关（P >0.05）。P-gp、GST-π在宫颈癌的阳性表达与以上临床病理特征无相关性（P >0.05）。③宫颈癌中Nek2与P-gp、GST-π表达呈正相关（r =0.193和0.207，P <0.05）。结论  Nek2可能介导宫颈癌中P-gp、GST-π的表达。Nek2、P-gp及GST-π蛋白在宫颈癌发生、发展及耐药机制中发挥重要作用。联合检测对宫颈癌治疗方案的合理制定及化疗反应性的评估可能具有积极的临床指导意义。

Abstract:

Objective To explore the expressions of never-in-mitosis A-related kinase 2 (Nek2), P-glycoprotein (P-gp) and glutathione S-transferase-π (GST-π) in cervical cancer, and analyze their correlations with clinical pathological features. Methods Immunohistochemistry was used to detect the expressions of Nek2, P-gp and GST-π in the cervical cancer tissues of 144 cases and chronic cervicitis tissues of 32 cases. Then the relationships of Nek2, P-gp and GST-π with clinicopathologic parameters were analyzed. Results The positive expression rate of Nek2, P-gp and GST-π in 144 cases of cervical cancer and 32 cases of chronic cervicitis tissues were 51.4% vs 31.2%, 55.6% vs 12.5% and 73.6% vs 53.1% respectively (P < 0.05). The  positive expression rate of Nek2 in the cervical cancer tissues was correlated to histological grade and clinical stages (P < 0.05), but not correlated with age, pathological type, depth of invasion, CK14, CEA, CK, P16 or survival time (P > 0.05). The positive expressions of P-gp and GST-π in the cervical cancers were not in correlation with the above clinicopathological features (P > 0.05). The expression of Nek2 in the cervical cancer was positively correlated with P-gp and GST-π expressions (r = 0.193 and 0.207, P < 0.05). Conclusions Nek2 maybe mediates P-gp and GST-π expressions in cervical cancer. Nek2, P-gp and GST-π play vital roles in the occurrence, development and drug-resistant mechanism of cervical cancer.