葡萄籽原花青素对自发性高血压大鼠主动脉重构的改善作用研究

Effect of grape seed procyanidins on improving aortic remodeling in spontaneous hypertension rats

摘要:

目的  探讨葡萄籽原花青素（GSP）对自发性高血压大鼠（SHR）主动脉重构的改善作用。方法  8周龄雄性SHR 24 只随机分为4组，每组6只：SHR组、GSP低剂量（50 mg/kg）、高剂量组（200 mg/kg）以及卡托普利组（30 mg/kg）。6只同龄雄性Wistar-Kyoto大鼠设为对照组。每周测定尾动脉收缩压（SBP）；治疗6周后HE染色下测定胸主动脉中膜横截面积（MCSA）、管腔面积（LA）及MCSA/LA；Masson三色法测量胸主动脉胶原容积分数（CVF）；ELISA法测定胸主动脉中Ⅰ型胶原（ColⅠ）含量；生化法检测大鼠胸主动脉中过氧化氢酶（CAT）活力及丙二醛（MDA）含量；蛋白免疫印迹法（Western blot）检测胸主动脉中ERK1/2蛋白表达。结果  GSP能显著降低SHR SBP（P <0.01），改善血管重构参数，减少胸主动脉MDA含量以及ERK1/2蛋白的表达，提高CAT活力。结论  GSP能够改善SHR胸主动脉重构，其作用可能是通过降压、对抗氧化应激和抑制ERK1/2蛋白表达实现的。

Abstract:

Objective To investigate the effect of grape seed procyanidins (GSP) on improving aortic remodeling in spontaneous hypertension rats (SHR). Methods Twenty-four male SHRs (8-week old) were randomized into 4 groups with 6 in each group: SHR group, low-dose GSP and high-dose GSP groups (50 or 200 mg/kg) and Captopril group (30 mg/kg). Meanwhile, 6 Wistar Kyoto rats (8-week old) were served as control group. Tail systolic pressure was measured every week. After 6-week treatment, media cross-sectional area (MCSA), lumen area (LA) and MCSA/LA of thoracic aorta were calculated after HE staining. Collagen volume fraction (CVF) was examined by Masson staining. ELISA and biochemical techniques were performed to determine the type I collagen (Col I), malondiadehyde (MDA) and catalase (CAT) levels respectively. Western blot was used to detect the expression of ERK1/2 in thoracic aorta. Results GSP could significantly decrease tail systolic pressure (P < 0.01), improve vascular remodeling parameters, reduce MDA content and the expression of ERK1/2 in thoracic aorta (P < 0.05), but increase CAT activity (P < 0.05) after 6-week treatment. Conclusions GSP significantly improves thoracic aorta remodeling of SHR by lowering systolic pressure, inhibiting oxidative stress as well as suppressing the expression of ERK1/2.