生殖支原体16SrRNA基因和MgPa基因TaqMan荧光聚合酶链反应检测的比较研究

Comparative study of TaqMan fluorescence PCR assay detection of mycoplasma genitalium by 16SrRNA gene and MgPa gene

摘要:

目的  对生殖支原体（Mg）16SrRNA基因和MgPa基因TaqMan荧光聚合酶链反应（PCR）检测结果进行比较，选取敏感度更高的靶基因进行TaqMan荧光PCR检测。方法  选取Mg 16SrRNA基因和MgPa基因为靶基因，根据已报道文献设计合成特异性扩增引物和探针，对泌尿生殖道拭子标本进行TaqMan荧光PCR检测，对Mg不同靶基因TaqMan荧光PCR检测结果进行统计学分析。结果  Mg 16SrRNA基因TaqMan荧光PCR检测敏感性为90%，MgPa基因TaqMan荧光PCR检测敏感性为97.5%。结论  MgPa基因TaqMan荧光PCR敏感性要高于16SrRNA基因TaqMan荧光PCR。

Abstract:

Objective To compare the TaqMan fluorescence PCR detection results between mycoplasma genitalium 16SrRNA gene and MgPa gene. Methods Mycoplasma genitalium 16SrRNA gene and MgPa gene were selected as target genes. According to the reported literatures, the specific amplified primers and probes were designed and synthesized. Urinary tract swab specimens were detected by TaqMan fluorescent PCR. The results of TaqMan fluorescence PCR detection of different target genes of Mycoplasma genitalium were statistically analyzed. Results The detection sensitivity of 16SrRNA gene and MgPa gene of mycoplasma genitalium TaqMan PCR was 90% and 97.5%, respectively. Conclusions The sensitivity of TaqMan PCR in the genital mycoplasma MgPa gene is higher than that of the 16SrRNA gene.