胆固醇结石症患者与正常人胆汁骨桥蛋白抑成核活性的比较

Comparison of anti-nucleation activity of biliary osteopontin from cholesterol gallstone patients and normal individuals

摘要:

目的  比较胆固醇结石症患者与正常人胆汁骨桥蛋白（OPN）抑成核活性及浓度的差异。方法  利用超速离心法结合凝胶电泳从胆固醇结石症患者及正常人胆汁中分离、纯化OPN蛋白。通过HOLAN法测定两组OPN的成核时间及成核活性。通过ELISA法检测胆固醇结石症患者与正常人胆汁中OPN浓度差异。结果  胆固醇结石症患者胆汁OPN蛋白较正常人具有较短的成核时间[（17.7±1.07）d vs （20.4±1.90）d]，较弱地抑制胆固醇结晶成核活性；且胆固醇结石症患者胆汁OPN蛋白浓度低于正常人胆汁OPN蛋白浓度[（69.60±5.41）ng/ml vs （82.08±5.63）ng/ml]，差异均有统计学意义（P <0.05）。结论  与正常人比较，胆固醇结石症患者胆汁OPN蛋白抑制胆固醇成核活性较弱、浓度较低。

Abstract:

Objective To compare cholesterol nucleation inhibiting activity and concentration of biliary osteopontin (OPN) protein from patients with cholesterol gallstone disease and normal individuals. Methods Biliary OPN protein were separated and purified from cholesterol gallstone patients and normal individuals using ultracentrifugation method combined with gel electrophoresis. Nucleation time and nucleation activity of OPN protein from the two groups were determined by HOLAN method. Concentration of biliary OPN protein of cholesterol gallstone patients and normal individuals were detected by Elisa method. Results Compared with normal individuals, the biliary OPN protein of cholesterol gallstone patients had shorter nucleation time (17.7±1.07 vs 20.4±1.90) d, and weaker cholesterol crystal nucleation inhibiting activity. Biliary OPN protein concentration in cholesterol gallstone patients were lower than in normal individuals (69.60±5.41 vs 82.08±5.63)ng/ml, the differences were statistically significant (P < 0.05). Conclusions Comparing with normal individuals, cholesterol gallstone patients have lower biliary OPN concentration and weaker cholesterol nucleation inhibiting activity.