枸杞多糖对精神分裂症模型大鼠海马中GluR1表达及认知功能的影响

Effect of Lycium barbarrum polysaccharides on hippocampal GluR1 and cognitive function of schizophrenia rats

摘要:

目的  探讨枸杞多糖（LBP）对精神分裂症模型（Sz）大鼠海马中GluR1表达及认知功能影响。 方法  选取36只健康SD大鼠，随机分3组，每组12只，分别为正常对照组、模型组及药物干预组（Sz+LBP）。Sz及Sz+LBP腹腔注射地卓西平马来酸盐0.6 mg/（kg·d），连续注射14 d制造精神分裂症模型，Sz+LBP同时灌胃枸杞多糖100 mg/（kg·d）。正常对照组在相应时段注射等剂量的正常生理盐水。造模成功后，分别评估各组大鼠的行为学改变，进行Morris水迷宫，比较3组大鼠的定位航行和空间探索时间以及采用免疫荧光染色、Western blot法测定海马中GluR1蛋白的表达。结果  Sz的运动量、共济失调、刻板行为评分以及海马中GluR1均高于正常对照组（P <0.01），Sz+LBP的运动量、共济失调及刻板行为评分均低于Sz，明显抑制精神分裂症大鼠海马中GluR1的表达（P <0.01）。结论  枸杞多糖对精神分裂症模型大鼠海马具有保护作用，其机制与海马中GluR1表达来改善其认知功能相关。

Abstract:

Objective To explore the effect of Lycium barbarrum polysaccharides (LBP) on receptor 1 of glutamic acid (GluR1) in hippocampus and cognitive function of schizophrenia rats. Methods Thirty-six SD rats were randomly divided into 3 groups with 12 in each group: normal group, model group and LBP group. Model group and LBP group were given MK801 (0.6 mg/kg·d) with sinistro-intraperitoneal injection to set the schizophrenia model. At the same time, LBP group was administered intragatrically with 100 mg/kg·d LBP. The control group was injected with 0.9% saline (NS) in the corresponding period. The behavioral changes of each rat were separately assessed with Morris experiment, and the navigation and time space exploration were compared between the three groups. Using immunofluorescence and Western blot, the GluR1 protein expression in hippocampus was determined. Results The model group had higher amount of exercise, ataxia, stereotyped behavior score and GluR1 expression in hippocampus than the control group (P < 0.01). LBP group had significantly lower amount of exercise, ataxia, stereotyped behavior score and GluR1 expression in hippocampus than the model group (P < 0.01). Conclusions LBP plays a protective role for schizophrenia, its mechanism may be due to down-regulation of GluR1 expression in the hippocampus of schizophrenia rats.