MicroRNA-124 rs531564、microRNA-26A rs7372209和microRNA-126 rs4636297多态性与结直肠癌易感性关联研究

Correlation analysis between miR-124 rs531564、miR-26A rs7372209 and miR-126 rs4636297 polymorphisms and susceptibility to colorectal cancer

摘要:

目的  探讨microRNA-124 rs531564（miR-124 rs531564）、microRNA-26A rs7372209（miR-26A rs7372209）和microRNA-126 rs4636297（miR-126 rs4636297）基因多态性与结直肠癌遗传易感的关系。 方法  利用聚合酶链式反应-连接酶检测反应（PCR-LDR）技术，对724例结直肠癌患者和626例健康对照个体的miR-124 rs531564、miR-26A rs7372209和miR-126 rs4636297多态位点进行分型。采用非条件逻辑回归分析统计该多态位点与结直肠癌易感的相关性，计算相对危险度的比值比（■）及95%置信区间（CI）。结果  该试验中miR-126 rs4636297多态位点，在病例中经调整年龄、性别、吸烟、饮酒等影响相对于CC基因型个体，发现携带AG或AA的基因型个体与结直肠癌的患病风险有一定的相关性（P <0.05)，调整后的■（95%）分别为[1.289（1.007，1.650）和1.383（0.396，4.834）]。相对于G等位基因，A等位基因显著提高个体患结直肠癌的风险（■=1.307，95%CI=1.054，1.622，P =0.013）；还发现携带A等位基因（AG+AA）的个体患结直肠癌风险是携带GG基因型个体的1.327倍（AG+AA vs GG，■=1.327，95%CI=1.042，1.688，P =0.019）。而miR-124 rs531564、miR-26A rs7372209位点的多态性并未发现不同基因型个体与结直肠癌的患病风险相关（P >0.05）。结论  miR-126 rs4636297基因多态性与结直肠癌的遗传易感有密切关系，携带A等位基因可能是促进结直肠癌发病风险的重要因素。

Abstract:

Objective To investigate the miR-124 rs531564, MiR-26A rs7372209 and MiR-126 rs4636297 gene polymorphism and colorectal cancer genetic susceptibility. Methods Polymerase chain reaction-ligase detection reaction (PCR-LDR) technology was used to detect 724 cases of colorectal cancer patients and 626 healthy control individuals of miR-124 rs531564, MiR-26A RS7372209 and MiR-126 RS4636297 polymorphism point type. Unconditional logistic regression analysis correlationwas used to calculate the relative risk of the statistical ratio of polymorphic loci and colorectal cancer susceptibility ratio (■) and 95% confidence intervals (CI). Results The test MiR-126 RS4636297 polymorphic loci, in cases adjusted for age, sex, smoking, alcohol consumption and other factors with respect to the CC genotype, showed that a certain correlation between the AA or AG genotype and colorectal cancer (P < 0.05), ■ (95%) were adjusted {1.289 (1.007, 1.650) and 1.383 (0.396, 4.834)} with respect to the G allele. A allele significantly increased individual risk of colorectal cancer risk (■=1.307, 95%CI = 1.054, 1.622, P = 0.013). The study also found that the risk of colorectal cancer for A allele (AG+AA) individuals was 1.327 times of GG genotype (AG+AA vs GG, ■= 1.327, 95%CI = 1.042, 1.688, P = 0.019). No correlation between the miR-124 rs531564, MiR-26A rs7372209 polymorphism loci and colorectal cancer risk (P > 0.05). Conclusions MiR-126 rs4636297 polymorphism and colorectal cancer is closely related to genetic susceptibility, and A allele might promote the incidence of colorectal cancer important risk factors.