肝细胞癌组织中TLR4、MyD88、NF-kB表达量及其与临床病理特征的相关性分析

Correlations of TLR4, MyD88 and NF-κB expressions with clinicopathological features in hepatocellular carcinoma

摘要:

目的  研究肝细胞癌组织中TLR4、MyD88、NF-κB表达量及其与临床病理特征的相关性。方法  选择肝细胞癌患者并采集血清、肝细胞癌组织、癌旁组织，选择健康志愿者并采集血清，检测肝脏组织中TLR4、MyD88、NF-κB的mRNA含量及血清中AFP、TK1、GP73、ASPH的含量。结果  肝细胞癌组织中TLR4、MyD88、NF-κB的mRNA含量均高于癌旁组织；TNM III-IV期、低未分化、淋巴结转移的肝细胞癌组织中，TLR4、MyD88、NF-κB的mRNA含量均高于TNM I～II期、高中分化、无淋巴结转移的肝细胞癌组织；肝细胞癌患者血清中AFP、TK1、GP73、ASPH的含量高于健康对照组，且与肝细胞癌组织中TLR4、MyD88、NF-κB的mRNA含量呈正相关。结论  肝细胞癌组织中TLR4通路功能增强且与肝细胞癌的临床病理分期、血清肿瘤标志物含量密切相关。

Abstract:

Objective To investigate the correlations of TLR4, MyD88 and NF-κB expressions with clinicopathological features in hepatocellular carcinoma. Methods Patients with hepatocellular carcinoma were chosen and their serum, hepatocellular carcinoma tissues and paracancerous tissues were collected, and serum was also collected from healthy volunteers. The mRNA levels of TLR4, MyD88 and NF-κB in hepatocellular carcinoma tissues and serum content of AFP, TK1, GP73 and ASPH were detected. Results The mRNA levels of TLR4, MyD88 and NF-κB in the hepatocellular carcinoma tissues were significantly higher than those of the paracancerous tissues. The mRNA levels of TLR4, MyD88 and NF-κB in the hepatocellular carcinoma tissues of the patients with TNM stage III-IV, poorly- differentiated or undifferentiated cancer and lymph node metastasis were significantly higher than those in the hepatocellular carcinoma tissues of the patients with TNM stage I-II, well-moderately differentiated cancer and no lymph node metastasis. The serum content of AFP, TK1, GP73 and ASPH in the hepatocellular carcinoma patients was significantly higher than that in the control group and positively correlated with TLR4, MyD88 and NF-κB mRNA levels. Conclusions In hepatocellular carcinoma, TLR4 pathway function is enhanced and closely related to clinicopathological stage and content of serum tumor markers.