白藜芦醇对人前列腺癌细胞株PC-3增殖及TGF-β1/Smad2信号通路影响研究

Effect of resveratrol on proliferation and TGF-β1 signaling pathway on human prostate cancer cell line PC-3

摘要:

目的  探讨白藜芦醇对前列腺癌细胞株PC-3增殖及转化生长因子β1（TGF-β1）/Smad2信号通路的影响。方法  不同浓度白藜芦醇干预体外培养前列腺癌细胞株PC-3白藜芦醇不同时间。MTT检测白藜芦醇对PC-3细胞生长情况的影响，流式细胞术检测细胞周期的变化情况，逆转录-聚合酶链反应（RT-PCR）和蛋白免疫印迹法（Western blot）分别检测细胞内TGF-β1和Smad2 mRNA和蛋白表达水平的变化情况。  结果  MTT结果显示白藜芦醇显著抑制了前列腺癌细胞的生长。流式细胞结果表明白藜芦醇明显降低了前列腺癌细胞S期含量而增加了G0/G1期含量。而TGF-β1和其下游因子Smad2的mRNA和蛋白水平的表达也明显受到白藜芦醇的抑制，且这种抑制作用呈浓度一时间依赖性。结论  白藜芦醇可能够通过阻断TGF-β1/Smad2信号通路抑制前列腺癌细胞生长前列腺癌。

关键词:

白藜芦醇;转化生长因子β1;前列腺癌

Abstract:

Objective To observe the effect of resveratrol on proliferation and TGF-β1 signaling pathway of human prostate cancer cell line PC-3. Methods Human prostate cancer cell line PC-3 was cultured and treated with resveratrol at different concentrations and different time points． The effect of resveratrol on PC-3 cell proliferation was studied by means of MTT. The cell cycle was detected with flow cytometry. RT-PCR and Western blot were used to detect the expressions of TGF-β1 and Smad2 at mRNA and protein levels. Results The viability of Huh-7 cells treated with resveratrol was obviously decreased. Analysis of the cell cycle revealed that resveratrol induced a significant decrease in cells in the S phase and increase in cells in the G1 phase. Furthermore, the expressions of TGF-β1 and its downstream factor-Smad2 were inhibited at mRNA and protein levels by resveratrol in a concentration- and time-dependent manner. Conclusions Resveratrol perhaps plays inhibitory effect on PC-3 cells through inhibiting the activity of TGF-β1 signaling pathway.

Key words:

resveratrol; TGF-β1 signaling pathway; prostate cancer