慢性乙肝患者血中细胞毒性T淋巴细胞表面PD-1/PD-L1的表达与病情的相关性

Correlation of expression of PD-1/PD-L1 on cytotoxic T lymphocytes in the peripheral blood of patients with chronic hepatitis B and disease severity

摘要:

目的  研究慢性乙型肝炎患者外周血中细胞毒性T淋巴细胞表面程序性死亡分子-1（PD-1）/程序性死亡分子-1配体（PD-L1）的表达及其与病情的相关性。方法  选择慢性乙型肝炎患者90例和健康体检者82例进行研究。分别纳入慢性乙肝组和健康对照组，检测外周血细胞毒性T淋巴细胞表面PD-1、PD-L1的表达量以及血清生化指标。结果  慢性乙肝组患者外周血中细胞毒性T淋巴细胞表面PD-1和PD-L1的表达量显著高于健康对照组，乙型肝炎病毒载量越高、外周血中细胞毒性T淋巴细胞表面PD-1和PD-L1的表达量越高；慢性乙肝组患者血清中ALT、AST、IL-6、IL-8的含量均显著高于健康对照组且与外周血中细胞毒性T淋巴细胞表面PD-1和PD-L1的表达量呈正相关。结论  慢性乙型肝炎患者外周血中细胞毒性T淋巴细胞表面PD-1和PD-L1的表达量显著升高且与肝损伤程度、炎症反应程度相关。

Abstract:

Objective To study the expression of PD-1/PD-L1 on cytotoxic T lymphocytes in the peripheral blood of patients with chronic hepatitis B and its correlation with disease severity. Methods A total of 90 cases of chronic hepatitis B patients and 82 cases of healthy subjects were respectively enrolled into the chronic hepatitis B group and the healthy control group. Expressions of PD-1 and PD-L1 on cytotoxic T lymphocytes in the peripheral blood and serum biochemical indices were determined. Results Expressions of PD-1 and PD-L1 on cytotoxic T lymphocytes in the peripheral blood of the chronic hepatitis B group were significantly higher than those in the healthy control group. Patients with higher HBV load also had a higher expression of PD-1 and PD-L1 on cytotoxic T lymphocytes in the peripheral blood. And serum ALT, AST, IL-6, IL-8 contents of chronic hepatitis B groups were significantly higher than those in healthy control group and positively correlated with PD-1 and PD-L1 expression on cytotoxic T lymphocytes in the peripheral blood. Conclusions The expression of PD-1 and PD-L1 on cytotoxic T lymphocytes in the peripheral blood of patients with chronic hepatitis B significantly elevates and correlates with the degree of liver injury and the degree of inflammation.