胎盘组织缺氧诱导因子-1α和内皮素-1表达对糖尿病妊娠的影响

Influence of placental hypoxia inducible factor-1α and endothelin-1 in gestational diabetes patients

摘要:

目的  探究分析胎盘组织缺氧诱导因子-1α（HIF-1α）和内皮素-1（ET-1）表达对糖尿病妊娠的影响。方法  分别选取糖尿病妊娠患者30例（糖尿病妊娠组）、妊娠期糖尿病患者30例（妊娠糖尿病组）以及正常孕妇30例（对照组）。采用免疫组织化学法SP法测定EF-1及HIF-1α蛋白的含量，比较并分析各组检测结果差异及其对糖尿病妊娠结局的影响。结果  糖尿病妊娠患者胎盘组织EF-1及HIF-1α蛋白阳性表达率显著高于妊娠糖尿病患者及对照组（P <0.05），糖尿病妊娠患者的产后出血、胎儿生长受限及妊娠高血压等并发症发生率（50.00%）显著高于妊娠糖尿病患者（30.00%）及对照组（10.00%），各组比较差异有统计学意义（P < 0.05）。结论  胎盘组织EF-1及HIF-1α蛋白表达情况对糖尿病妊娠患者的妊娠结局具有不良影响，EF-1及HIF-1α蛋白阳性表达率越高，妊娠并发症发生率明显增加，胎盘损害越严重。

Abstract:

Objective To explore the the influence of placental hypoxia inducible factor 1 alpha (HIF-1α) and endothelin-1 (ET-1) in gestational diabetes. Methods A number of 30 patients diabetes during pregnancy and 30 gestational diabetes patients were selected, and 30 cases of normal pregnant women were selected at the same time as a control group. Immune histochemical method and SP method were used to detect EF-1 and HIF-1α protein content. Differences between groups of test result and its influence on pregnancy outcomes of diabetes were compared and analysis. Results The placental tissue EF-1 and HIF-1α protein positive expression rate in patients with diabetes during pregnancy was significantly higher than that of gestational diabetes mellitus patients and the control group (P < 0.05). Patients with diabetes during pregnancy had higher rate of postpartum hemorrhage, fetal growth restriction and higher incidence of complications (P < 0.05). Conclusions The expressions of EF-1 and HIF-1α protein in placental tissue have adverse effects on patients with diabetes during pregnancy.