雌激素受体α、雌激素受体β、激活蛋白1、血管内皮生长因子在大鼠子宫内膜异位症模型中的表达及意义

Expression and significance of estrogen receptors α and β, activator protein and VEGF in rat model of endometriosis

摘要:

目的  探讨雌激素受体α（ERα）、雌激素受体β（ERβ）、激活蛋白1（AP-1）、血管内皮生长因子（VEGF）在大鼠子宫内膜异位症（EMs）模型中的表达及意义。方法  采用皮下种植法建立大鼠EMs模型，选取建模成功的28只大鼠为模型组（n =28），观察大鼠EMs模型在位内膜及异位病灶的组织病理学改变，并通过免疫组织化学法检测对照组子宫内膜、模型组在位子宫内膜和异位病灶ERα、ERβ、AP-1、VEGF的表达情况。结果  ①大鼠EMs模型在位内膜及异位病灶腺体、间质血管明显增加；②大鼠EMs模型的异位病灶与对照组子宫内膜间ERα阳性率比较，差异无统计学意义（P =0.107），而其在位内膜中的表达较对照组比较，差异有统计学意义（P <0.01）。ERβ、AP-1（c-jun）蛋白、VEGF在模型组在位内膜及异位病灶中阳性率与对照组比较，差异有统计学意义（P <0.01），但其在位内膜及异位病灶中的表达比较，差异无统计学意义。结论  大鼠EMs模型在位内膜中ERα和ERβ均高表达，异位病灶中则仅表现为ERβ高表达，同时上调c-jun，VEGF的表达，ERβ可能在EMs的发生发展中起到较ERα更为重要的作用。

Abstract:

Objective To explore the expression and significance of estrogen receptor α (ERα), ERβ, activator protein 1 (AP-1) and VEGF in rat model of endometriosis (EM). Methods  Subcutaneous implantation was used to establish EM model in female rats. The rats with successful ectopic implants were served as model group (n = 28), and the normal rats served as control group (n = 10). The histopathological changes of ectopic implants were observed after four weeks. The expression levels of ERα, ERβ, AP-1 and VEGF in the endometrium and endometriosis lesions were observed by immunohistochemistry in the two groups. Results There were more glands and blood vessels in the stroma of endometriosis lesions and the endometrium of the model group. There was no statistically significant difference in ERα expression level between the endometriosis lesions of the EM model and the endometrium of the control group (P = 0.107), but its expression level in the endometrium of the model group was obviously increased compared with the control group (P < 0.01). The expression levels of ERβ, AP-1 and VEGF in the endometriosis lesions and endometrium of the EM model were obviously increased compared with those of the control group (P < 0.01), but there was no statistically significant difference between the endometriosis lesions and endometrium of the EM model. Conclusions ERα and ERβ are obviously increased in the endometrium of the EM rat model; but in the endometriosis lesions, only ERβ is significantly increased. Moreover, the expressions of AP-1 and VEGF are up-regulated in the EM model. ERβ may play a more important role than ERα in the occurrence of endometriosis.