不同品系小鼠肺纤维化模型的比较研究

Comparative study on pulmonary fibrosis model induced by Bleomycin in three strains of mice

摘要:

目的  通过对博来霉素诱导3个不同品系小鼠肺纤维化程度的比较研究，寻找最适合研究肺纤维化的小鼠模型。方法  选用C57BL/6、BALB/c以及KM 3种品系的小鼠，分别设为模型组和对照组。模型组向气管注射博来霉素（5 mg/kg），正常对照组注入等量的生理盐水。3个品系的小鼠分别于造模后的第7天、14天和28天处死8只，观察在不同的时间点肺系数、羟脯氨酸含量的变化以及通过Masson染色观察肺纤维化程度的改变。结果  各模型组不同时间点的肺系数、羟脯氨酸含量和肺组织的病理学改变积分与对照组比较，差异有统计学意义（P <0.05）；C57BL/6模型组小鼠在不同时间点肺系数、羟脯氨酸含量和肺组织病理学改变积分与BALB/c和KM比较，差异有统计学意义（P <0.05）。结论  C57BL/6小鼠对博来霉素反应敏感，肺纤维化明显，是3个品系小鼠中研究肺纤维化的最理想模型。

Abstract:

Objective To find out the most proper model of human pulmonary fibrosis through a comparative study of pulmonary fibrosis in three strains of mice induced by Bleomycin(BLM). Methods C57BL/6, BALB/c and KM mice were randomly divided into model groups and normal control groups, respectively. Three model groups received a single intratracheal instillation of 5 mg/kg body weight of BLM to induce pulmonary fibrosis, while normal control groups were administrated the same amount of normal saline. On the 7th, 14th and 28th day, 8 mice were respectively sacrificed in each model group. Lung tissues were removed to measure the lung coefficient and hydroxyproline content of the mice at different time points, and Masson staining was used to evaluate the extent of pulmonary fibrosis. Results The lung coefficient, hydroxyproline content and pulmonary histopathological changes of each model group were remarkably different from those of the corresponding normal control group (P < 0.05). Compared with the BALB/c and KM model groups, the lung coefficient, hydroxyproline content and pulmonary histopathological changes of the C57BL/6 model group were significantly increased (P < 0.05). Conclusions C57BL/6 is more suitable for the model of pulmonary fibrosis in the three strains of mice because of sensitive Bleomycin reaction and obvious pulmonary fibrosis. Of the three strains, C57BL/6 is the most suitable model in studying pulmonary fibrosis.