牙周膜干细胞膜片构建的生物性牙根生物学活性研究

Biological activity of biological roots constructed by periodontal ligament stem cell sheet

摘要:

目的  将经过鉴定的人牙周膜干细胞（hPDLSC）膜片分别与支架材料预处理牙本质片（TDM）和羟基磷灰石/磷酸三钙（HA-TCP）进行体外共培养，评价不同支架对干细胞分化的影响。方法  将单层牙周膜干细胞膜片与2种不同支架材料TDM和HA-TCP分别共培养1周，植入裸鼠皮下，通过扫描电镜和组织学动态观察复合体显微结构变化。结果  细胞膜片与TDM边界处结合更为紧密；边界大量矿物沉积和纤维样组织生成；而在HA-TCP组只有单一的走形相对规则的纤维组织生成，未有明显矿化沉积生成。结论  复合的hPDLSC/TDM组更适合具有生物学特点的干细胞发挥其特性，在合适的微环境下使hPDLSC向成牙周组织方向定向分化。

Abstract:

Objective To co-culture the identified human periodontal ligament stem cell membranes (hPDLSC) respectively with pretreated dentin matrix (TDM) and hydroxyapatite /tricalcium (HA-TCP) in vitro, and evaluate the effects of different scaffolds on stem cell differentiation. Methods Monolayer periodontal ligament stem cell membrane was incubated with two different scaffold materials for 1 week, and implanted into osteoporosis rats. Using scanning electron microscopy and histological section, the microsture changes were dynamically observed. Results A lot of mineral deposition and fibrous tissue were formed in the dentin matrix (TDM) group and there was some irregular fibrous tissue regeneration in the HA-TCP group without obvious mineral material generation. Conclusions hPDLSC/TDM composite is more suitable for the stem cells to fully develope their own biological characteristics. It can be more suitable for hPDLSC to differentiate into periodontium in suitable microenvironment.