河南省部分地区人群散发戊型肝炎流行病学及病毒基因型分析

Epidemiological and genotype analysis of sporadic Hepatitis E in some parts of Henan

摘要:

目的  研究分析河南省南阳地区散发戊型肝炎的流行病学特征及流行毒株的基因型。方法  在对目标人群的戊型肝炎流行病学调查过程中，通过酶联免疫吸附试验（ELISA）对采集到的血清样本进行抗体检测，对其中抗体为阳性的戊型肝炎病例，通过巢式PCR（nested RT-PCR）方法，扩增基因组ORF2区域150 bp的片段并进行测序及生物信息学分析。结果  本研究采集到的1 100份血清样品中有295份血清戊肝抗体阳性，阳性率26.8%。男女戊肝患病比为1.86︰1，戊肝患者中男性多于女性，80.3%的患者集中在35岁及以上的年龄组。HEV RNA检测表明128份血清阳性，阳性率43.4%，病毒序列在分型上属于基因4型，与中国西安株的核苷酸序列同源性最高。结论  南阳地区的戊肝以散发为主，基因4型是该地区的优势基因型。本地区戊肝的亲缘关系与中国西安株最近，推断两地毒株可能来源于戊肝病毒的同一种系。

Abstract:

Objective To investigate the epidemiological characteristics of the sporadic Hepatitis E infection cases in Nanyang area. Methods In the investigation process of the target population, ELISA was used to detect the antibodies in the collected serum samples. The 150bp fragments of the ORF2 were amplified from the positive samples by nested RT-PCR and the target fragments were sequenced and analyzed. Results 295 of 1100 serum samples were HEV antibody positive, and the positive rate was 26.8%. Sporadic hepatitis E cases of 80.3% occurred with age over 35, and the male to female ratio was 1.86 : 1. A total of 128 serum samples were HEV RNA positive, and the viral sequences belonged to the genotype 4 with the maximum homology to Xi'an isolate. Conclusions Hepatitis E sporadic in Nanyang area and genotype 4 is the predominant strain in the city. The strains isolated in Nanyang have the closest relationship with Xi'an isolate, and this maybe infer that these two strains probably originate from the same HEV strain.