缺氧对前列腺癌细胞糖酵解及迁移侵袭能力的影响

Hypoxia regulates glycolysis and metastasis of prostatic cancer in vitro

摘要:

目的  探讨缺氧状态下前列腺癌细胞糖酵解和体外迁移侵袭能力的改变。方法  将前列腺癌细胞DU145和/或PC-3分别置于常氧及缺氧环境中培养24和48 h，侵袭小室实验检测前列腺癌细胞体外迁移及侵袭能力改变。分别检测上清液中葡萄糖含量、乳酸含量；实时定量逆转录-聚合酶链反应（qRT-PCR）检测糖酵解相关基因的表达改变。结果  前列腺癌细胞DU145经缺氧处理后，体外迁移及侵袭能力较常氧处理增强。同时缺氧处理后，DU145和PC-3细胞培养上清液中葡萄糖含量减少，肿瘤细胞摄入葡萄糖能力提高，糖酵解代谢产物乳酸在上清液中增加。qRT-PCR结果表明，缺氧处理后DU145细胞糖酵解相关基因。结论  缺氧处理能增强前列腺癌细胞的体外迁移及侵袭能力，同时通过改变糖酵解相关基因的表达，对前列腺癌细胞的糖酵解过程发挥调控作用。

Abstract:

Objective To investigate the changes of glycolysis and metastasis of prostatic cancer under hypoxia. Methods DU145 and PC-3 cells were cultured in normoxia and hypoxia for 24 hours and 48 hours, respectively. Then, Transwell invasion assay was used to detect the changes of migration and invasion. The content of glucose and lactate in the supernatant of each group was examined by commercial kits. Finally, genes associated with glycolysis were quantified by qRT-PCR. Results The migratory and invasive abilities of DU145 cells were significantly increased after hypoxia treatment. Meantime, compared with the cells cultured in normoxia, DU145 and PC-3 cells treated with hypoxia showed decreased supernatant glucose, enhanced glucose uptaking ability and lactate secretion. qRT-PCR results demonstrated that genes associated with glucose metabolism were correspondingly changed under hypoxia. Conclusions Hypoxia promotes the migration and invasion of prostatic cancer cells in vitro. Meantime, hypoxia can regulate the glycolysis of prostatic cancer cells via modulating expression of the genes associated with glycolysis.