脂肪干细胞与不同脱细胞基质的组织相容性研究

Histocompaticblity research of adipose stem cells with different acellular bladder matrix

摘要:

目的  研究SD大鼠脂肪干细胞与不同脱细胞膀胱基质（BAMG）的体外生物相容性，为进一步把细胞支架复合物回植动物体内提供有利的实验基础。方法  取SD大鼠双侧腹股沟处脂肪组织经过相应处理后得到原代脂肪组织，进行成骨、成脂诱导染色及流式细胞术检测CD29、CD34、CD44及CD45，对脂肪干细胞进行鉴定，同时制备鼠及新西兰兔BAMG，采用2次沉淀法将脂肪干细胞静态接种于不同BAMG表面，观察细胞在材料表面的生长状况，并绘制细胞生长曲线。结果  细胞材料体外复合培养5 d后行HE染色后观察可见SD大鼠脂肪干细胞在SD大鼠BAMG表面生长状态较其在新西兰兔BAMG表面生长状态好，且细胞形态舒展成长梭形，细胞生长曲线与SD大鼠原代脂肪干细胞基本一致。结论  SD大鼠脂肪干细胞接种于SD大鼠BAMG复合物表面生长状况相比于其接种于新西兰兔BAMG复合物表面更加良好，具有更好的生物相容性。为进一步把细胞支架复合物回植动物体内提供有利的实验基础。

Abstract:

Objective To study the in vitro biocompatibility of SD rat adipose stem cells with different acellular bladder matrix (bladder acellular matrix graft, BAMG), and provide favorable experimental basis to further implantation into animals. Methods In the bilateral inguinal adipose tissue of SD rats, the primary fat cells were obtained after treatment. CD29, CD34, CD44 and CD45 were used to identify adipose derived stem cells by staining and flow cytometry. BAMG of SD rats and New Zealand rabbits were prepared. Using secondary precipitation method, adipose stem cells were inoculated on different BAMG surface, cell growth on the surface of the materials was observed, and cell growth curve was drawn. Results After in vitro culture of the cellular materials for 5 days, HE staining revealed the adipose derived stem cells of SD rats grew better on the surface of SD rat BAMG than on the surface of New Zealand rabbit BAMG, the cells grew into spindle cells, and the cell growth curve was consistent with that of the primary fat cells of SD rats. Conclusions Adipose stem cells of SD rats grow better on the surface of SD rat BAMG complex than on the surface of New Zealand rabbit BAMG complex, it has better biological compatibility. Our result provides favorable experimental basis for further implantation of the cell scaffold complex into animals.