结直肠癌与溶质载体家族第5家族8基因甲基化相关性研究

Correlation of colorectal cancer and SLC5A8 gene methylation

摘要:

目的  研究结直肠癌组织中溶质载体家族第5家族8（SLC5A8）基因甲基化现象。方法  采集结直肠癌患者标本中的癌组织、癌旁组织和正常组织，应用甲基化特异性聚合酶链式反应（PCR）法检测SLC5A8基因甲基化水平。结果  结直肠癌组织、癌旁组织及正常组织中SLC5A8基因甲基化率，差异有统计学意义（P <
0.05），其中结直肠癌组织中SLC5A8基因甲基化阳性率与癌旁组织及正常组织之间差异有统计学意义（P <0.05）。SLC5A8的表达与其甲基化状态成负相关（r =-0.682）。结论  SLC5A8基因的甲基化是结直肠癌发生过程中的重要事件，与其发生、发展可能存在密切关联。

Abstract:

Objective To study the methylation of SLC5A8 gene in colorectal cancer. Methods Specimens adjacent to carcinoma tissue and normal tissue in the patients with colorectal cancer tissue were collected. Methylation specific polymerase chain reaction (PCR) method was used to detect SLC5A8 gene methylation level. Results Differences in colorectal cancer tissue, the tissue adjacent to carcinoma and normal tissues of SLC5A8 gene methylation rate were statistically significant (P < 0.05). Differences of SLC5A8 gene methylation positive rate in colorectal cancer tissues, cancer adjacent tissues and normal tissues was statistically significant (P < 0.05). The SLC5A8 gene expression and methylation status is negative correlation (r = -0.682). Conclusions The SLC5A8 gene methylation is important in the process of colorectal cancer occurs, it may have closely relationship of occurrence and development.