C-myc基因表达及细胞凋亡在脂溢性角化病皮损中的意义

Significance of C-myc expression and cell apoptosis in seborrheic keratosis

摘要:

目的  观察脂溢性角化病（SK）中C-myc基因表达及细胞凋亡情况，探讨脂溢性角化病的发病机制。方法  对66例脂溢性角化病皮损及10例正常皮肤组织采用免疫组织化学法检测C-myc基因表达及原位细胞凋亡检测（TUNEL）法检测细胞凋亡情况。结果  两组性别、年龄差异均无统计学意义（P >0.05）；SK组皮损区C-myc基因阳性细胞数百分比（21.94±19.45）%与对照组（0%）相比增加（P <0.05）；66例SK皮损中有52例C-myc基因表达阳性，对照组中均无阳性表达，两组阳性率差异有统计学意义（P <0.05）；TUNEL凋亡检测中SK组的凋亡指数（53.01±31.97）与对照组（33.50±23.86）比较，差异无统计学意义（P >0.05）；Spearman相关性分析显示，C-myc基因在SK皮损中阳性细胞百分比与SK皮损中细胞凋亡指数差异无统计学意义（P >0.05）。结论  C-myc基因参与脂溢性角化病的发生发展，脂溢性角化病皮损中细胞凋亡有所增加。该结果对研究其发病机制有一定的指导意义。

Abstract:

Objective To observe the expression of C-myc and apoptosis in seborrheic keratosis (SK) and investigate the pathogenesis of SK. Methods Imunohistochemistry was used to detect the expression of C-myc, and the apoptosis of cell were detected by TUNEL in 66 SK lesions and 10 normal controls. Results There was no significant difference in gender and age between the two groups(P > 0.05). The percentage of C-myc positive cells (21.94 ± 19.45) % in SK lesions were significantly higher than that in the control groups (0) (P  < 0.05). The protein of C-myc was positively expressed in 52 cases of the 66 SK lesions while none was expressed in the control groups (P < 0.05). There was no significant difference in apoptotic index between SK lesions (53.01 ± 31.97) and the control groups (33.50 ± 23.86) by TUNEL (P > 0.05). There were no significant correlation between the percentage of C-myc protein positive cells and the apoptosis index in SK lesions by spearman analysis (P > 0.05). Conclusions C-myc protein is involved in the occurrence and development of SK, and the apoptosis of SK is increased.