西妥昔单抗联合铂类化疗对人喉鳞癌Hep-2细胞裸鼠移植瘤COX-2及MMP-7的影响

Impact of Cox-2 and MMP-7 of cetuximab combined with cisplatin on human laryngeal neoplasms Hep 2 cells nude mice transplanted tumor

摘要:

目的  探讨西妥昔单抗联合铂类化疗对人喉鳞癌Hep-2细胞裸鼠移植瘤环氧化酶-2（COX-2）及基质金属蛋白酶-7（MMP-7）的影响。方法  选择BALB/c裸鼠24只为研究对象，均复制人喉鳞癌Hep-2细胞裸鼠移植瘤模型，随机分为西妥昔单抗组、顺铂组、西妥昔单抗联合顺铂组及对照组，各6只。对照组腹腔注射0.9%氯化钠溶液0.2 ml，西妥昔单抗组腹腔注射1 036 μg/ml西妥昔单抗注射液，顺铂组腹腔注射3μg/ml顺铂注射液，西妥昔单抗联合顺铂组腹腔注射1 036 μg/ml西妥昔单抗注射液和3 μg/ml顺铂注射液，连续4周后，脱颈处理裸鼠。比较瘤体生长情况、COX-2与MMP-7表达及信使核糖核酸（mRNA）表达。结果  西妥昔单抗组、顺铂组、西妥昔单抗联合顺铂组瘤体体积及瘤重均低于对照组（P <0.05），西妥昔单抗联合顺铂组瘤体体积、瘤重低于西妥昔单抗组与顺铂组，抑瘤率高于西妥昔单抗组及顺铂组（P <0.05）；西妥昔单抗组、顺铂组、西妥昔单抗联合顺铂组裸鼠肿瘤组织COX-2、MMP-7及mRNA表达均低于对照组（P <0.05），西妥昔单抗联合顺铂组裸鼠肿瘤组织COX-2、MMP-7表达及mRNA表达低于西妥昔单抗组、顺铂组（P <0.05）。结论  西妥昔单抗联合铂类化疗有助于抑制喉鳞癌Hep-2细胞裸鼠移植瘤增殖生长，可能与抑制肿瘤组织COX-2与MMP-7表达有关。

Abstract:

Objective To study effects of Cox-2 and MMP-7 of cetuximab combined with cisplatin on human laryngeal neoplasms Hep 2 cells nude mice transplanted tumor and its mechanism. Methods A total of 24 BALB/c human laryngeal neoplasms Hep 2 cells nude mice were established, and were divided into cetuximab group, cisplatin group, cetuximab combined cisplatin group, blank control group. The control group was treated with intraperitoneal injection 0.9% sodium chloride solution 0.2 ml, the cetuximab group with intraperitoneal injection 1,036 μg/ml cetuximab injection, cisplatin group with intraperitoneal injection 3 μg/ml cisplatin injection, and cetuximab combined with cisplatin group with intraperitoneal injection 1,036 μg/ml cetuximab injection and 3 μg/ml cisplatin injection. After four weeks, tumors growth, tumor tissue Cox-2 and MMP-7 protein expression and mRNA expression were compared. Results Volume and weight of tumors in the cetuximab group, cisplatin group, cetuximab combined cisplatin group were significantly lower than those in the control group (P < 0.05). Volume and weight of tumors in the cetuximab combined cisplatin group were significantly lower than those in cetuximab group, cisplatin group, inhibition rate were significantly higher cetuximab group, cisplatin group (P < 0.05). Expression of COX-2, MMP-7 protein and mRNA in cetuximab group, cisplatin group, cetuximab combined cisplatin group were significanlty lower than those in the control group (P < 0.05). Expressions of COX-2, MMP-7 protein and mRNA in cetuximab combined cisplatin group were significanlty lower than cetuximab group, cisplatin group (P < 0.05). Conclusions Cetuximab combination with cisplatin chemotherapy helps to inhibit laryngeal neoplasms Hep 2 cells nude mice transplanted tumor proliferation and growth. It may related to inhibiting tumor tissue Cox-2 and MMP-7 expression.