前列腺素E1后处理对大鼠移植肝缺血再灌注损伤的保护作用

Protection effect of post - processing prostagandin E1 in rat liver transplantation ischemia - reperfusion injury

摘要:

目的  探讨前列腺素E1（PGE1）后处理对大鼠移植肝缺血再灌注损伤（IRI）的保护作用及其机制。方法  建立SD大鼠肝移植模型，随机分为3组：IRI组、PGE1后处理组（PGE1组）及假手术组（S组）。检测各组血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、超氧化物岐化酶（SOD）及丙二醛（MDA）的含量；采用逆转录聚合酶链式反应（RT-PCR）方法检测移植肝组织内肿瘤坏死因子（TNF-α）信使核糖核酸（mRNA）的表达水平；苏木精-伊红染色（HE）观察移植肝病理形态学变化。结果  供肝再灌注后2及6 h，PGE1组的大鼠血清ALT、AST和MDA水平及TNF-α mRNA的表达低于IRI组（P <0.05），血清SOD水平高于IRI组（P <0.05），光镜下PGE1组肝组织损伤较IRI组轻。结论  PGE1后处理可通过提高肝组织的抗氧化能力，降低TNF-α来减轻大鼠移植肝IRI。

Abstract:

Objective To study the protective effects and mechanisms of prostaglandin E1 (PGE1) post-conditioning in liver graft ischemia-reperfusion injury. Methods The liver transplantation rat model was used as ischemia-reperfusion injury animal model. The rats were randomly divided into three groups: ischemic reperfusion injury group (IRI), postconditional PGE1 treated group (PGE1) and sham operated group (S). The liver functional tests, SOD, MDA, TNF-α expression level and the histology were checked at 2 h and 6 h after reperfusion. Results The ALT, AST, MDA in serum and TNF-α expression level in liver were significantly lower in PGE1 group than in IRI group (P < 0.05), the SOD was higher in the PGE1 group (P < 0.05). The tissue damage was significantly decreased in PGE1 group. Conclusions Prostaglandin E1 postconditioning has significant protective effect in ischemia-reperfusion injury, and part of those benefit effects dependent on decreased TNF-α expression level and increased SOD production.